GET THE APP

..

Veterinary Science & Technology

ISSN: 2157-7579

Open Access

Cholinesterase Effects of Pesticides in Rabbit Embryos and their Potential for Use in Hazard Assessment

Abstract

Kasim Sakran Abass* and Kamal Ali Salih

Cholinesterase are often taken as biomarkers of exposure and effect. Then acetylhydrolase isozymes and acylcholine acylhydrolase were examined for their suitability as biomarkers of pesticide exposure. The study of effects of embryos of the rabbit is already an accepted tool in monitor food waste levels, but effects of various groups of substances to the rabbit embryo remain to be studied. A 43% inhibition of acetylhydrolase enzyme activities was reached by 250 mg/kg malathion, while 35% inhibition of acylcholine acylhydrolase enzyme activities was reached by 500 mg/kg malathion indicating the possible use of these enzymes as biomarkers of exposure. There are found some significant specific activities of the acetylhydrolase ranged from 1.5-7.1 U mg−1 protein. The specific activity of acylcholine acylhydrolase in the range of 0.9-4.5 U mg−1 protein in the respective developmental stages. Substratespecificity was analyzed using specific inhibitors (iso-OMPA and BW284c51). The results showed that the observed cholinesterase’s activities in the whole embryo may be attributed mainly to acetylhydrolase with a partial capability to use butyrylthiocholine (BuSCh) as an additional substrate.

PDF

Share this article

Google Scholar citation report
Citations: 4472

Veterinary Science & Technology received 4472 citations as per Google Scholar report

Veterinary Science & Technology peer review process verified at publons

Indexed In

 
arrow_upward arrow_upward