Circulating Myeloid Dendritic Cells is Decreased in the Acute Phase of Kawasaki Disease
|Kenji Suda1,2*, Shintaro Kishimoto1, Tomoyuki Takahashi3, Yozo Teramachi1, Takato Yokoyama4, Hideo Yasukawa2, Keizo Ohbu4, Tsutomu Imaizumi2, Toyojiro Matsuishi1,3|
|1Department of Pediatrics and Child Health, Kurume University School of Medicine, Japan|
|2Cardiovascular Research Institute, Kurume University, Japan|
|3Cognitive and Molecular Research Institute of Brain Diseases, Kurume University, Japan|
|4Department of Pediatrics, St. Mary’s Hospital, Japan|
|Corresponding Author :||Kenji Suda, MD
Cardiovascular Research Institute
Kurume University School of Medicine
67 Asahi-Machi, 830-0011, Japan
E-mail: [email protected]
|Received September 10, 2013; Accepted October 07, 2013; Published October 10, 2013|
|Citation: Suda K, Kishimoto S, Takahashi T, Nishino H, Okamura H, et al. (2013) Circulating Myeloid Dendritic Cells is Decreased in the Acute Phase of Kawasaki Disease. J Clin Exp Cardiolog 4:272. doi: 10.4172/2155-9880.1000272|
|Copyright: © 2013 Suda K, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
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Background: Kawasaki disease is the most prevalent vasculitis of children in the developed countries that affects middle-sized arteries. Though T-cells are known to be activated with ample production of cytokines in acute phase of Kawasaki disease, there is a paucity of data concerning dendritic cells (DCs), the most potent antigen presenting cells that initiates T-cell activation. This study examined change in circulating DCs in acute phase of Kawasaki disease.
Methods: Using multi-color flow cytometry, we determined circulating myeloid DC (mDC), Lin-HLA-DR+CD11c+ cell, and plasmacytoid DC (pDC), Lin-HLA-DR+CD123+ cell in 33 patients with acute phase of Kawasaki disease (aKD), 24 febrile controls (FC), and 13 healthy controls (HC). Blood chemistry data including cytokines were determined at the same time. Numbers of DCs were compared among 3 groups and before and after immunoglobulin treatment in aKD. Correlation between numbers of circulating DCs and blood chemistry data were determined.
Results: Number of circulating mDC was significantly lower in aKD on admission than in FC and HC [median (lower, upper quartile)=7260 (2463, 11550) vs. 12210 (9500, 22050) and 18600 (11520, 23460) cells/ml, p < 0.001]. This number of circulating DCs significantly correlated with disease severity represented by serum albumin (mDC, r=0.56, p < 0.0001; pDC, r=0.39, p < 0.02, respectively), C reactive protein (mDC, r=-0.42, p < 0.005), and interleukin-6 (mDC, r=-0.55, p < 0.007). Immunoglobulin treatment quickly restored number of mDC [7260 (2463, 11550) vs. 15200 (10840, 30965) after IVIG and 18600 (12950, 25510) cells/ml at convalescence, p < 0.001] in aKD.
Conclusions: This study indicates that number of circulating mDCs is decreased in acute Kawasaki disease, and may be involved in the pathophysiology.