Cisplatin Induced Histological and Ultrastructural Alterations In Liver Tissue of Rat
- *Corresponding Author:
- Pratibha R.Kamble
17B, Kaupineshwar, Chendani Koliwada
Natu Paranjpee Colony, Near Siddhivinayak Mandir
Thane (East) 4000603, India
E-mail: [email protected]
Received Date: August 16, 2011; Accepted Date: October 17, 2011; Published Date: October 21, 2011
Citation: Kamble PR, Bhiwgade DA (2011) Cisplatin Induced Histological and Ultrastructural Alterations In Liver Tissue of Rat. J Cytol Histol 2:128. doi: 10.4172/2157-7099.1000128
Copyright: © 2011 Kamble PR, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: Cisplatin is well-known anticancer drug often been used for treatment of various human malignancies. The hepatotoxic or nephrotoxic effect of drug is a result of altered histological and antioxidant status of the organ. In order to document the extent of cisplatin (CDDP) effect on liver we have studied long term treatment of cisplatin (CDDP) to rat. In our previous studies we have demonstrated changes in reduced glutathione (GSH) and glutathione related enzymes of liver followed by increased lipid peroxidation process. Materials: Light microscopy (LM) was carried out in liver tissue by haematoxylin and eosin staining. Electron microscopy was performed by staining with uranil acetate and lead citrate. Results: Recent reports depicts that CDDP treatment caused significant alteration at histopathological level showing increased vacuolation in hepatocytes. A noticeable change observed after drug treatment is large perilobular connective and expanded portal spaces. Morphological alterations after transmission electron microscopy (TEM) showed heterochromatic border in nucleus followed by prescence of large agglutinations of lysosomes, numerous rough endoplasmic reticulum (RER) and mitochondria has been observed as one of the significant effect of drug. Conclusion: Thus cytoarchitectural studies signify that long term of CDDP intervention at 0.4 mg/kg/day/animal caused least damage to liver.