alexa Clinical Outcome of Autologous Cultivated Limbal Epithelial Transplantation Therapy for Patients with Ocular Surface Disease
ISSN: 2157-7633

Journal of Stem Cell Research & Therapy
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Clinical Outcome of Autologous Cultivated Limbal Epithelial Transplantation Therapy for Patients with Ocular Surface Disease

Umapathy T1*, Alias R1, Lim MN2, Alagaratnam J1, Zakaria Z2 and Lim TO3

1Department of Ophthalmology, Kuala Lumpur Hospital, Malaysia

2Institute for Medical Research, Kuala Lumpur, Malaysia

3Virtuallux Sdn Bhd, Kuala Lumpur, Malaysia

*Corresponding Author:
Dr. Thiageswari Umapathy, MBBS (Mysore)
MSOphth (National University of Malaysia)
Consultant of Ophthalmologist, Department of Ophthalmology
Hospital Kuala Lumpur, 50588 Jalan Pahang, Kuala Lumpur, Malaysia
Tel: +60326162714
E-mail: [email protected]

Received date: October 22, 2014; Accepted date: November 20, 2014; Published date: November 22, 2014

Citation: Umapathy T, Alias R, Lim MN, Alagaratnam J, Zakaria Z, et al. (2014) Clinical Outcome of Autologous Cultivated Limbal Epithelial Transplantation Therapy for Patients with Ocular Surface Disease. J Stem Cell Res Ther 4:248. doi: 10.4172/2157-7633.1000248

Copyright: © 2014 Umapathy T, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



 

Abstract

Introduction: Cultivated Limbal Epithelial Cells Transplantation (CLET) has been proven effective in reconstructing cornea surface of Limbal Stem Cell Deficiency (LSCD). To better understanding the potential and risk of this treatment in our current setup, we conducted a phase I clinical trial and determined the outcome of cultivated limbal epithelial transplantation for Ocular Surface Disease (OSD).
Methods: Prospective interventional trial was conducted in Hospital Kuala Lumpur, Malaysia. Fourteen eyes of 14 patients with limbal stem cells deficiency: chemical injury (6), advanced pterygium (4), advanced Vernal Kerato Conjunctivitis (VKC) (2), Persistent Epithelial Defect (PED) (1) and Ocular Cicatricial Pemphygoid (OCP) (1) were selected according to the inclusion/exclusion criteria. Autologous cornea limbal epithelial stem cells were cultured on human amniotic membrane and subjected to immunohistological staining for a panel of markers (ABCG2, cytokeratins (K) 3, K19, p63, involucrin, integrin α9 and K14). Cell sheets with epithelial morphology and growth area greater than 80% will be used for CLET. Two weeks later, the tissue was transplanted to the recipient eyes after superficial keratectomy. The patients were followed up for 1 year. Outcome measures were improvement of symptoms, an improvement in visual acuity, no conjunctivalisation and vascularisation and healed persistent corneal epithelial defect.
Results: Ten patients (71.4%) had an improvement in visual acuity of at least two lines at 6 months and one year (p<0.05%). Thirteen patients (92.8%) had cornea epithelisation in 1 month. The patient with persistent epithelial defect (PED) healed in 3 months and the patient with ocular cicatricial pemphygoid (OCP) had recurrent cornea epithelial defect at 6 months and healed after one year. On slit lamp examination, eight patients (57.1%) had no cornea vascularisation in 6 months and 1 year (p<0.05%). Ten patients (71.4%) had no cornea conjunctivalisation in 6 months and 1 year (p<0.05%).
Conclusions: Transplantation of cultivated autologous limbal epithelial cell can efficiently restore the cornea with improved vision. Patients with chemical injury, advance pterygium group had better outcome results.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]omicsonline.com

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords