alexa Cloning and Expression of Truncated Chlamydial Major Outer Membrane Protein in E.coli: A Miniature Step Forward
ISSN: 2157-7560

Journal of Vaccines & Vaccination
Open Access

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Research Article

Cloning and Expression of Truncated Chlamydial Major Outer Membrane Protein in E.coli: A Miniature Step Forward

Razieh Bitazar1,2, Taghi Naserpour Farivar1*, Bahareh Hajikhani2*, Rezvan Bagheri2 and Ali Salimi3

1Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran

2Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran

3Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran

*Corresponding Author:
Taghi Naserpour Farivar
Cellular and Molecular Research Center
Qazvin University of Medical Sciences, Qazvin, Iran
Tel: +98 281 3336002; +98 21 22432020
E-mail: [email protected]
Bahareh Hajikhani
Reproductive Biotechnology Research Center
Avicenna Research Institute, ACECR, Tehran, Iran
E-mail: [email protected]

Received date: December 21, 2013; Accepted date: January 25, 2014; Published date: January 28, 2014

Citation: Bitazar R, Farivar TN, Hajikhani B, Bagheri R, Salimi A (2014) Cloning and Expression of Truncated Chlamydial Major Outer Membrane Protein in E.coli: A Miniature Step Forward. J Vaccines Vaccin 5:217. doi: 10.4172/2157-7560.1000217

Copyright: © 2014 Bitazar R, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Administration of Prophylactic vaccines still is the cutting-edge of prevention, control and elimination of infections. Among the common causes of sexually transmitted diseases, chlamydia trachomatis is an important socioeconomic burden worldwide which impairs human genital tract function, resulting in severe complications like urethritis, pelvic inflammatory disease (PID), getting ease of HIV transmission, playing cofactor role in human papilloma virus (HPV)- induced cervical neoplasia, and unfortunately infertility. Because of all difficulties, vaccination is considered to be the most economical and reliable way to escape from unrestrained exacerbation states of infection than any other prevalence program. But despite much advancement in the field of veterinary, the dream has yet to be realized. Among a number of potential candidates, the major outer membrane protein (MOMP) is a vanguard of subunit vaccines. In this attempt, MOMP216 gene fragment cloned in PET28b+ and expressed in E.coli. The protein expression confirmed by SDS-PAGE. These findings demonstrate that, considering the antigenicity prediction and due to the existence of potential epitopic region, rMOMP216 could be a potential capable peptide which can represent as an alternative to whole MOMP.

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