CLONING OF HUMAN ERYTHROPOIETIN GENE IN pVAX1 VECTOR FOR PRODUCTION OF r-DNA epo
The epo.hu gene is already cloned in pTarget vector. In the present study we have released the epo.hu gene insert from the pTarget.epo.hu using plasmid isolation, RE digestion and ligated with pVAX1 vector. Prepared the competent cell to transformation of ligated product in E.coli DH5α. Make the L.B Agar plate and the large number of colonies (approximately 25) were picked from the overnight grown transformants. The individual colonies were inoculated in fresh ampicillin (50 μg/ml) containing LB broth and allowed to grow for large scale production for further experiments. This can be used for expression and immunological studies.