Comparative Effects of Scorpion Venom and Aqueous Basil (Ocimum basilicum) Leaves Extracts on Ccl4-induced Toxicity in Albino Rats
Muhammad MA Salman*, Naglaa RA Kasem and Nora HM Saleh
Department of Zoology, South Valley University, Qena, Egypt
- *Corresponding Author:
- Muhammad MA Salman
Faculty of Science
Department of Zoology
South Valley University
Received date: August 25, 2016; Accepted date: September 19, 2016; Published date: September 27, 2016
Citation: Salman MMA, Kasem NRA, Saleh NHM (2016) Comparative Effects of Scorpion Venom and Aqueous Basil (Ocimum basilicum) Leaves Extracts on Ccl4-induced Toxicity in Albino Rats. J Liver 5:201 doi: 10.4172/2167-0889.1000201
Copyright: © 2016 Salman MMA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The present study aims to compare the therapeutic effects between to extract scorpion venom (bradykinin potentiating factor; BPF) and aqueous extract of Sweet basil (Ocimum basilicum) on CCl4-induced toxicity of liver and kidney in albino rats. Rats were divided into 8 groups. Group (1) was served as normal group; Group (2) was injected intraperitoneal (i.p.) once a week for 3 weeks with BPF at a dose (1 μgm/g). Group (3) was received orally O. basilicum extract, twice a week for 6 weeks at dose (20 ml/kg). Group 4 was treated with the same doses of BPF and O. basilicum. Group (5) was injected i.p. with CCl4 (1 ml/kg), 3 times weekly, for 2 weeks and served as control group. Groups 6, 7 and 8 were injected i.p. with CCl4 then treated i.p. with BPF, orally with O. basilicum and BPF plus O. basilicum respectively. The results of the present study cleared that normal group (1) and groups (2, 3 and 4) showed no significant difference in all liver and kidney functions, besides antioxidant enzymes except creatinine and GSH, were highly significant increase in group (4). CCl4 caused a highly significant decrease in serum albumin, uric acid, besides Catalase, GSH, SOD activities in liver tissue, while there are an elevation in serum AST, ALT, ALP, γ-GT, creatinine and Urea, besides MDA and NO levels in liver tissue. While, groups (6, 7 and 8) revealed reverse effect in all parameters and recorded a remarkable improvement, comparing with normal group. It can be concluded that the treatment with the extract from the scorpion venom Buthus occitanus (BPF) is more effective than those of extract from the plant (O. basilicum) against the toxicity of liver and kidney-induced by CCl4 in albino rats. In addition, the hepato-ameliorating and antioxidant effects of two extracts were found to be better than those of extract of BPF or O. basilicum indepently.