Comparative Evaluation of Growth Media for the Cultivation of Fungal Cultures
- *Corresponding Author:
- Chinyerum Gloria Ikechi – Nwogu
Department of Plant Science & Biotechnology Faculty of Science
University of Port Harcourt, Nigeria
E-mail: [email protected]
Received date: September 12, 2012; Accepted date: October 27, 2012; Published date: October 30, 2012
Citation: Ikechi–Nwogu CG, Elenwo EN (2012) Comparative Evaluation of Growth Media for the Cultivation of Fungal Cultures. J Plant Pathol Microb 3:139. doi: 10.4172/2157-7471.1000139
Copyright: © 2012 Ikechiâ€“Nwogu CG, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
An evaluation was carried out on some growth media for the cultivation of fungal cultures. Fungi such as
Aspergillus niger, Aspergillus flavus, Penicillium chrysogenum, Aspergillus terreus, Aspergillus glaucus, Fusarium oxysporium, and Rhizopus stolonifer were isolated from food materials using Standard Blotter Method. Pure cultures were obtained and transferred into Potato Dextrose, Soybean Dextrose, Sawdust Sucrose, Ofor (Detarium macrocarpum) Sucrose and Groundnut Dextrose Broths respectively, to screen their suitability for culturing fungi. After incubation at 27 ± 1°C for 7 days, the organisms were harvested, weighed with a weighing balance to measure their growth rate. The mean values for all the organisms in Soybean broth except Fusarium oxysporium, were significantly (P ≤ 0.05) higher than those of Groundnut Dextrose, Ofor (Detarium macrocarpum) Sucrose Broth, Sawdust Sucrose Broth and Potato Dextrose Broth. Soybean Dextrose Broth, performed better than other broths probably because it contains more vitamins and minerals vital to fungal growth.