Comparative Lipid Isolation Protocols from Rhizophora mucronata L. of Dharmadam Estuary and Lipid Profiling in the Context of a Non Mangrove Mangifera indica
Meera SP, Anusha S and Anu A*
Department of Biotechnology and Microbiology, Kannur University, Thalassery Campus, Palayad P.O., Kannur- 670661, Kerala, India
- *Corresponding Author:
- Anu Augustine
Department of Biotechnology and Microbiology
Kannur University, Thalassery Campus
Palayad P.O., Kannur-670661, Kerala, India
Tel: +91 9447151040
E-mail: [email protected]
Received date: May 13, 2015; Accepted date: August 22, 2016; Published date: August 26, 2016
Citation: Meera SP, Anusha S, Anu A (2016) Comparative Lipid Isolation Protocols from Rhizophora mucronata L. of Dharmadam Estuary and Lipid Profiling in the Context of a Non Mangrove Mangifera indica. Biochem Anal Biochem 5:286. doi:10.4172/2161-1009.1000286
Copyright: © 2016 Meera SP, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The effects of salt stress on lipid composition of mature mangrove plant, Rhizophora mucronata grown in estuarine water (0.391 M Chloride ion concentration and 3020 ppm salinity) and yield of total lipids from two original protocols and four modified protocols were evaluated. It is the first approach for isolation of mangrove lipids, in which propanol treatment was used, which resulted in two fold increase in lipid yield. Rhizophora mucronata lipid composition is categorized into seven prominent classes: Polar lipids, Unknown, Sterols, Free fatty acids, Tri-terpenoids, Wax esters and Sterol esters. Comparative study of mangrove lipid profile (Rhizophora mucronata control and Rhizophora mucronata mature) with that of a salt sensitive tree Mangifera indica showed that in Rhizophora mucronata plants grown in high saline conditions, the concentration of sterol esters, wax esters and sterols get decreased and tri-terpenoids, free fatty acids, unknown lipids and polar lipids get increased.