alexa Comparative Proteomics and Network Analysis Identify PKC Epsilon Underlying Long-Chain Fatty Acid Signaling
ISSN: 0974-276X

Journal of Proteomics & Bioinformatics
Open Access

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Research Article

Comparative Proteomics and Network Analysis Identify PKC Epsilon Underlying Long-Chain Fatty Acid Signaling

Tomo Yonezawa1,2,3*, Riho Kurata1,3,4, Atsushi Tajima1, Xiaofeng Cui5, Hiroki Maruta3, Hirofumi Nakaoka1, Keiichi Nakajima6 and Hidetoshi Inoko1

1Division of Basic Medicine Science and Molecular Medicine, School of Medicine, Tokai University, Bohseidai, Ishehara, Kanagawa 259-1193, Japan

2Department of Pharmacology and Therapeutic Innovation, Nagasaki University, Graduate School of Biomedical sciences, 1-14 Bunkyo-machi, Nagasaki 852-8531, Japan

3Research Institute for Biomedical Sciences, Tokyo University of Science, 2669 Yamazaki, Noda, Chiba 278-0022, Japan

4Japan Society for the Promotion Science, Japan

5School of Chemistry, Chemical Engineering and Life Science, Wuhan University of Technology, 122 Luoshi Road, Wuhan, Hubei 430070, China

6NARO Hokkaido Agricultural Research Center, Hitsujigaoka 1, Toyohira, Sapporo 062-8555, Japan

*Corresponding Author:
Tomo Yonezawa
Division of Basic Medicine Science and Molecular Medicine
School of Medicine, Tokai University, Bohseidai
Ishehara, Kanagawa 259-1193, Japan
Tel: +81-4-7121-4105
Fax: +81-4-7121-4104
E-mail: [email protected];[email protected]; [email protected]

Received Date: September 10, 2014; Accepted Date: October 09, 2014; Published Date: October 14, 2014

Citation: Yonezawa T, Kurata R, Tajima A, Cui X, Maruta H, et al. (2014) Comparative Proteomics and Network Analysis Identify PKC Epsilon Underlying Long-Chain Fatty Acid Signaling. J Proteomics Bioinform 7: 340-346. doi: 10.4172/jpb.1000337

Copyright: © 2014 Yonezawa T, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

Abstract

Long-chain fatty acid possesses myriad roles in the biological function of the cells, not only as an energy substrate but also as substrates for cell membrane synthesis and as precursors for intracellular signaling molecules. However, little is known about the biological pathways that are stimulated by long-chain fatty acid. In order to identify the pathway of long-chain fatty acid, we performed 2-dimensional gel electrophoresis in the cells treated with or without oleate, and then analyzed 648 protein spots using PDQuest software and narrowed down 22 significant changing spots by statistical criterion. We also tried to determine these spots by MALDI-QIT-TOF-MS and SWISSPROT database query. We identified 11 proteins and predicted the biological network using available data sets from protein-protein interaction database. This prediction indicated that several protein kinase Cs (PKCs) underlie longchain fatty acid signaling. Indeed, oleate stimulated predicted PKC pathways. In expression array, oleate significantly up-regulated only PKC epsilon, but not other PKCs, in transcriptional levels. Collectively, our proteomics and network analysis implicates that PKC epsilon pathway plays an important role in long-chain fatty acid signaling.

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