alexa Comparative Studies on Performance of CCC and Preparative RP-HPLC in Separation and Purification of Steroid Saponins from Dioscorea Zingiberensis C.H.Wright
ISSN: 2157-7536

Journal of Steroids & Hormonal Science
Open Access

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Research Article

Comparative Studies on Performance of CCC and Preparative RP-HPLC in Separation and Purification of Steroid Saponins from Dioscorea Zingiberensis C.H.Wright

Xinxin Zhang1, Jianli Liu1, Wenji Sun1 and Yoichiro Ito2*

1Biomedicine Key Laboratory of Shaanxi Province, Northwest University, 229 Taibai North Road, Xi’an, Shaanxi 710069, China

2Laboratory of Bioseparation Technology, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA

*Corresponding Author:
Yoichiro Ito
Laboratory of Bioseparation Technology
Biochemistry and Biophysics Center
National Heart, Lung, and Blood Institute
National Institutes of Health, Bethesda, Maryland, USA
Tel: +1 (301) 496-1210
Fax: +1 (301) 402-0013 E-mail: [email protected]

Received date: December 29, 2014; Accepted date: February 19, 2015; Published date: February 25, 2015

Citation: Zhang X, Liu J, Sun W, Ito Y (2015) Comparative Studies on Performance of CCC and Preparative RP-HPLC in Separation and Purification of Steroid Saponins from Dioscorea Zingiberensis C.H.Wright. J Steroids Hormon Sci 6:150. doi:10.4172/2157-7536.1000.150

Copyright: © 2015 Zhang X, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

SteroidSaponins from Dioscorea zingiberensis C.H.Wright were separated for the first time using two chromatographic methods for comparison: counter-current chromatography (CCC) coupled with evaporative light scattering detector (ELSD) and preparative reversed phase high-performance liquid chromatography (RP-HPLC) with an ultraviolet detector. Ethyl acetate-n-butanol-methanol-water (4:1:2:4, v/v) was chosen as the two-phase solvent system for CCC, while the acetonitrile-water (25:75 for the first step and15:85 for the second step, v/v) was used as the mobile phase in the preparative RP-HPLC. The following five steroid Saponins were purified by these two chromatographic methods, in one-step operation by CCC and by two-step operation in preparative RP-HPLC:

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