alexa Comparative Study of Prions in Iatrogenic and Sporadic Creutzfeldt-Jakob Disease | OMICS International | Abstract
ISSN: 2155-9899

Journal of Clinical & Cellular Immunology
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Comparative Study of Prions in Iatrogenic and Sporadic Creutzfeldt-Jakob Disease

Xiangzhu Xiao1#, Jue Yuan1#, Liuting Qing1, Ignazio Cali1,10, Jacqueline Mikol2, Marie-Bernadette Delisle3,4, Emmanuelle Uro-Coste3,4, Liang Zeng1,9, Mai Abouelsaad1, Dimitris Gazgalis1, Manuel Camacho Martinez1, Gong-Xian Wang9, Paul Brown5, James W. Ironside6, Pierluigi Gambetti1, Qingzhong Kong1,7and Wen-Quan Zou1,7,8,9*
1Department of Pathology and National Prion Disease, Pathology Surveillance Center, Case Western Reserve University, 2085 Adelbert Road, Cleveland, Ohio 44106, USA
2Department of Pathology, Hôpital Lariboisière, 2 rue Ambroise Paré, Paris, France
3Department of Pathology , Rangueil University Hospital, avenue Jean Poulhes, TSA 50032, 31059 Toulouse Cedex 9, France
4INSERM U858, I2MR, Team 15, BP 84225, 31432 Toulouse Cedex 4, France
5Commissariat à 1’Énergie Atomique (CEA), Institute of Emerging Diseases and Innovative Therapies, Fontenay-aux-Roses, France
6National Creutzfeldt-Jakob Disease Surveillance Unit, Western General Hospital Edinburgh, EH4 2XU, United Kingdom
7Department of Neurology, Case Western Reserve University, 2085 Adelbert Road, Cleveland, Ohio 44106, USA
8National Center for Regenerative Medicine, Case Western Reserve University, 2085 Adelbert Road, Cleveland, Ohio 44106, USA
9The First Affiliated Hospital, Nanchang University, Nanchang, Jiangxi Province, The People’s Republic of China
10Department of Clinical and Experimental Medicine, Second University of Naples, Naples, Italy
#These authors contributed to this work equally
Corresponding Author : Wen-Quan Zou, M.D., Ph.D.
Institute of Pathology
Case Western Reserve University School of Medicine
2085 Adelbert Road, Cleveland, OH 44106, USA
Tel: +1-216-368-8993
Fax: +1-216-368-8993
E-mail: [email protected]
Received: June 16, 2014; Accepted: July 22, 2014; Published: July 29, 2014
Citation: Xiao X, Yuan J, Qing L, Cali I, Mikol J, et al. (2014) Comparative Study of Prions in Iatrogenic and Sporadic Creutzfeldt-Jakob Disease. J Clin Cell Immunol 5:240. doi:10.4172/2155-9899.1000240
Copyright: © 2014 Xiao X, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Related article at
DownloadPubmed DownloadScholar Google

Abstract

Differentiating iatrogenic Creutzfeldt-Jakob disease (iCJD) from sporadic CJD (sCJD) would be useful for the identification and prevention of human-to-human prion transmission. Currently, the diagnosis of iCJD depends on identification of a recognized source of contamination to which patients have been exposed, in addition to fulfilling basic requirements for the establishment of diagnosis of CJD. Attempts to identify differences in clinical manifestations, neuropathological changes and pathological prion protein (PrPSc) between iCJD and sCJD have been unsuccessful. In the present study, using a variety of more sophisticated methods including sucrose step gradient sedimentation, conformational stability immunoassay, protein misfolding cyclic amplification (PMCA), fragment-mapping, and transmission study, we show no significant differences in gel profiles, oligomeric state, conformational stability and infectivity of PrPSc between iCJD and sCJD. However, using PMCA, we find that convertibility and amplification efficiency of PrPSc is greater in iCJD than in sCJD in a polymorphism-dependent manner. Moreover, two protease-resistant PrP C-terminal fragments (termed PrP-CTF12/13) were detected in all 9 cases of sCJD but not in 6 of 8 cases of iCJD tested in this study. The use of fragment mapping- and PMCA-based assays thus provides a means to distinguish most cases of iCJD from sCJD.

Keywords

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

kactakapaniyor.com

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

Taktube

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

porn sex

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

Gaziantep Escort

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

sikiş

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

https://www.gaziantepescort.info

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

mp3 indir

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

putlockers

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

seks

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version