alexa Comparative Study on Separation of Proteins from Whey W
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
Open Access

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Research Article

Comparative Study on Separation of Proteins from Whey Waste by Solvent Sublation in Batch and Continuous Mode

Samadrita Saha1, Nasim Sepay2, Pranjal Das3, Sarmisttha Kundu3, Milan Kumar Maiti3 and Goutam Mukhopadhyay3*

1Calcutta Institute of Pharmaceutical Technology and Allied Health Science, Howrah, West Bengal, India

2Department of Chemistry, Presidency University, West Bengal, India

3BCDA College of Pharmacy and Technology, Kolkata, West Bengal, India

*Corresponding Author:
Goutam Mukhopadhyay
BCDA College of Pharmacy and Technology
Kolkata, West Bengal-700 127, India
Tel: +919836333563
E-mail: [email protected]

Received date: November 25, 2016; Accepted date: December 09, 2016; Published date: December 15, 2016

Citation: Saha S, Sepay N, Das P, Kundu S, Maiti MK, et al. (2016) Comparative Study on Separation of Proteins from Whey Waste by Solvent Sublation in Batch and Continuous Mode. J Chromatogr Sep Tech 7:343. doi: 10.4172/2157-7064.1000343

Copyright: © 2016 Saha S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

The work is based on investigation on the separation of proteins from whey waste collected from local confectionery. Separation phenomenon involves solvent sublation technique both in batch and continuous mode. The objective of this work was to make a comparative study of afore mentioned batch and continuous method. The effect of pH, initial feed, concentration of feed, nitrogen gas flow rate, surfactant concentration, ionic strength, concentration of organic solvent and temperature were investigated in details. The amount of protein recovered in single continuous mode after 5 hr was 3644 mg, %Rp (90%), total volume (8100 ml), effective concentration (49.5 mcg/ml). The VFR 14 cc/min, %Rp was nearly 1.3 times more in continuous solvent sublation technique in comparison to the batch mode at optimum pH and GFR.

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