Comparison between DNA and Analyses Morphometrics as Tool for Identification of Sex of the Golden Eagles (Aquila chrysaetos Canadensis)Gerardo Guerra-Paramo1, Fernando Garcia-Gil1*, Oscar Escobedo-Correa2 and Fidel de la Cruz Hernandez-Hernandez3
- *Corresponding Author:
- Fernando Garcia-Gil
Universidad Simón Bolívar
Escuela de Biología, Mexico
Tel: +58 212-906311
E-mail: [email protected]
Received date: January 22, 2015; Accepted date: April 28, 2015; Published date: April 30, 2015
Citation: Guerra-Paramo G, Garcia-Gil F, Escobedo-Correa O, Hernandez- Hernandez FC (2015) Comparison between DNA and Analyses Morphometrics as Tool for Identification of Sex of the Golden Eagles (Aquila chrysaetos Canadensis). Entomol Ornithol Herpetol 4:151. doi:10.4172/2161-0983.1000151
Copyright: © 2015 Guerra-Paramo G, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The Golden eagle (Aquila chrysaetos canadensis) is an endangered species in Mexico, and the captive breeding is relevant to its conservation. Monomorphic raptor species, such as Golden eagle, are difficult for sex determination based only on morphometric measures and plumage characteristics, and a reliable method to determine the sex of birds is important for conservation strategies. With the purpose of forming new captive breeding couples, the sex of 10 adult Golden eagles was identified using DNA analysis such as CHD gene amplification (P8, P2 primers), and by CHD amplification by ARMS technique. In addition bioinformatic, and PCA statistical analysis of morphometric characteristics were conducted. We confirmed that the P2-P8 pair of primers inside the CHD gene was not a good option for Golden eagles because fragments derived from sex specific alleles CHD-W and CHD-Z differed just in 6 bp, which are not enough to be distinguished in agarose gel electrophoresis. In contrast, sex identification in Golden eagles with ARMS technique was fast, reliable and useful, obtaining a difference of more than 60 bp between CHD-W and CHD-Z that allows discriminating directly the alleles in a simple agarose gel. The PCA analysis of morphometric characteristics indicated three significant morphological patterns that differentiated males from females.