Comparison of 5-Aminolaevulinic Acid and its Heptyl Ester-induced Protoporphyrin IX and its Photobleaching in Human Adenocarcinoma WiDr Cells and in Athymic Nude Mice Healthy Skin
Xiao Pudroma* and Gongsangduoji
Tibet University, 850000 Lhasa, Tibet, People’s Republic of China
- *Corresponding Author:
- Xiao Pudroma
Department of science, Tibet University
850000 Lhasa, Tibet
People‘s Republic of China
Tel: +86- 13889016338
E-mail: [email protected]
Received date: April 19, 2012; Accepted date: May 21, 2012; Published date: May 24, 2012
Citation: Pudroma X, Gongsangduoji (2012) Comparison of 5-Aminolaevulinic Acid and its Heptyl Ester-induced Protoporphyrin IX and its Photobleaching in Human Adenocarcinoma WiDr Cells and in Athymic Nude Mice Healthy Skin. J Nucl Med Radiat Ther 3:131. doi:10.4172/2155-9619.1000131
Copyright: © 2012 Pudroma, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
5-aminolevulinic acid heptyl ester (heptyl 5-aminolevulinate), a potential prodrug for fluorescence diagnosis and Photodynamic Therapy (PDT), was investigated in human adenocarcinoma WiDr cells and in healthy skin of athymic nude mice in comparison with 5-aminolevulinic acid (ALA). Incubation of WiDr cells with ALA and ALA heptyl ester resulted in the production of Protoporphyrin IX (PpIX). Concentrations higher than 0.01 mM for ALA heptyl ester and 1 mM for ALA were cytotoxic. Similar amounts of PpIX production and same photobleaching rate were observed in WiDr cells, although a 100 times lower concentration of ALA heptyl ester was needed in comparison with ALA. In contrast to cells culture, topical application of 2% of ALA and ALA heptyl ester gave similar fluorescence of PpIX and PpIX photobleaching in mouse model. But a faster PpIX pbotobleaching was observed in vivo compared to in vitro.