alexa Comparison of Analytical Methods to Detect Polysialic A
ISSN: 2153-0637

Journal of Glycomics & Lipidomics
Open Access

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Research Article

Comparison of Analytical Methods to Detect Polysialic Acid

Saki Nishimura1, 2, Masaya Hane1,2, Yuki Niimi1,2, Shinji Miyata3, Ken Kitajima1,2 and Chihiro Sato1,2*

1Bioscience and Biotechnology Center, Nagoya University, Chikusa Nagoya 464-8601, Japan

2Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa Nagoya 464-8601, Japan

3Institute for Advanced Research, Nagoya University, Chikusa Nagoya 464-8601, Japan

*Corresponding Author:
Chihiro Sato
Bioscience and Biotechnology Center
Nagoya University, University, Chikusa
Nagoya 464-8601, Japan
Tel: 81-52-789- 4295
Fax: 81-52-789-5228
E-mail: [email protected]

Received date: April 18, 2014; Accepted date: April 19, 2014; Published date: May 31, 2014

Citation: Nishimura S, Hane M, Niimi Y, Miyata S, Kitajima K, Sato C (2014) Comparison of Analytical Methods to Detect Polysialic Acid. J Glycomics Lipidomics 4:113. doi: 10.4172/2153-0637.1000113

Copyright: © 2014 Nishimura S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Polysialic acid (polySia) is a highly anionic polymer of sialic acid that mostly modifies the neural cell adhesion molecule
(NCAM), and involved in brain functions such as learning, memory, circadian rhythm, and social behaviors, through making
repulsive and attractive fields on the cell surface. Recently, polySia has been reported to have relationships with some
diseases such as psychiatric disorders and cancers. To understand functions of polySia and to apply evaluation methods
of polySia structures to the diagnosis of diseases, the comparison of the methods for determining the quality and quantity
of polySia structures would be important and necessary. In this study, two monoclonal antibodies, 12E3 and 735, of distinct
binding properties were chosen for immunochemical methods by an enzyme-linked immunosorbent assay (ELISA) and a
Western-blotting. Two chemical methods, mild acid hydrolysis-fluorometric anion exchange chromatography (MH-FAEC)
analysis, and fluorometric C7/C9 analysis were also chosen. These methods were applied to a small amount of crude adult
and embryonic brain homogenates to evaluate the quantity and quality of polySia structure. The quantity of polySia can
be evaluated using anti-polySia antibodies by ELISA. In addition, MH-FAEC can be also applied to the evaluation of both
quantity and quality (chain length) of polySia chain. Biochemical characters of polySia-NCAM in crude homogenates can
be analyzed using a mono Q-anion exchange chromatography. Combinational analyses of these methods could help our
further understanding of polySia structure, and the quantity and quality of polySia will become criteria useful for diagnosis
of diseases.

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