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ISSN: 2327-5073

Clinical Microbiology: Open Access
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Research Article

Comparison of Bacterial Composition in Blood and Placentas Using Conventional and Molecular Methods

Tingtao Chen1,2*, Xin Wang2, Qinglong Wu1, Meixiu Jiang2, Kan Deng2, Caibin Zhang2, Fengcai Zhang2, Shaoguo Yang2, Ling Mo2, Yi He2 and Hua Wei1*

1State Key Laboratory of Food Science and Technology, Nanchang University, Jiangxi, China

2Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi 332000, PR China

*Corresponding Author:
Dr. Hua Wei
Professor, Jiangxi-OAI Joint Research Institute
Nanchang University, 235 Nanjing Donglu
Nanchang, Jiangxi, 330047, P.R. China
Tel: +86-791-88334578
Fax: +86-791-88333708
E-mail: [email protected]
 
Dr. Tingtao Chen
Associate Research Professor
Institute of Translational Medicine
Nanchang University, 1299 Xuefu Road, Nanchang
Jiangxi, 330047, P.R. China
Tel: +86-791-88334578
E-mail: [email protected]

Received date: April 25, 2015; Accepted date: May 16, 2015; Published date: May 23, 2015

Citation: Chen T, Wang X, Wu Q, Jiang M, Deng K, et al. (2015) Comparison of Bacterial Composition in Blood and Placentas Using Conventional and Molecular Methods. Clin Microbiol 4:200. doi: 10.4172/2327-5073.1000200

Copyright: © 2015 Chen T, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Fetus was considered to be sterile and little work is done to explore the ignored microbial diversity in it. To investigate the bacterial diversity in the feces, blood and placentas of pregnant mice and to assess the translocation capability of the administered strains, conventional culture and PCR denaturing gradient gel electrophoresis (PCRDGGE) were used in the present study. The culture method detected microbes (lactobacilli, enterobacter and enterococcus) in the blood and in the placentas at positive rates of 27.8%, 55.6% and 11.1% and 22.2%, 66.7% and 16.7%, respectively. The PCR-DGGE results showed that the E. faecalis, L. lactis and an uncultured bacterium were the dominant bacteria found in the feces, blood and placentas, and the transport of E. faecalis FD3 from the intestinal tract to the blood and to the placenta was also observed, revealing a potential risk to the health of both mother and fetus. In conclusion, the combination of classical cultivation and PCR-DGGE methods provides a superior strategy for the fast and accurate monitoring of the microbial composition in the blood and in the placentas of pregnant mice.

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