alexa Comparison of ELISA, Antigenemia Assay and Nested PCR Monitoring Techniques for Detection of Cytomegalovirus Infection in Renal Transplantation Patients| Abstract
ISSN: 2161-0703

Journal of Medical Microbiology & Diagnosis
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  • Research Article   
  • J Med Microb Diagn 2018, Vol 7(1): 274
  • DOI: 10.4172/2161-0703.1000274

Comparison of ELISA, Antigenemia Assay and Nested PCR Monitoring Techniques for Detection of Cytomegalovirus Infection in Renal Transplantation Patients

Ehsan Moghanloo1,2, Vahid Babaei3, Soheila Rezaei4, Leila Khanmirzaei5, Tina Delsouz Bahri6, Hamidreza Zeraatgar Gohardani7,8 and Shahram Teimourian3*
1Faculty of Medicine, Department of Microbiology and Immunology, Kashan University of Medical Sciences, Kashan, Iran
2Department of Infectious Diseases, School of Medicine, Pediatric Infectious Diseases Research Center, University of Medical Sciences, Tehran, Iran
3Department of Medical Genetics, Iran University of Medical Sciences (IUMS), , Tehran, Iran
4Department of Molecular Genetics, National Institute of Genetic Engineering and Biotechnology, , Tehran, Iran
5Department of Biology, Islamic Azad University, Arak Branch, Arak, Iran
6Faculty of Medicine, Department of Microbiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran
7Department of Virology, Pasture Institute of Iran, , Tehran, Iran
8Baharloo Hospital, Tehran University of Medical Sciences (TUMS), , Tehran, Iran
*Corresponding Author : Shahram Teimourian, Department of Medical Genetics, Iran University of Medical Sciences, Crossroads Of Shahid Hemmat And Shahid Chamran Highways, P. O. Box: 15875-6171 Tehran, Iran, Tel: 00982186703243, Fax: 00982188602209, Email: [email protected]

Received Date: Feb 05, 2018 / Accepted Date: Feb 19, 2018 / Published Date: Feb 23, 2018

Abstract

Human cytomegalovirus (HCMV) can be transmitted through blood transfusion and organ transplantation and could be cause of some complication in solid-organ transplant recipients. Current study is aimed to compare the sensitivity and Specificity of ELISA, Antigenemia assay and nested PCR methods to detection of Cytomegalovirus infection in renal transplantation patients.

In this study blood samples were collected from 200 renal transplant recipients’ patients.

DNA was extracted by commercial kit and Nested PCR was done by 2 pairs of internal and external primers. Anti CMV antibodies (IgM and IgG) were detected by ELISA and CMV-pp65 antigenemia assay (Ag) was used to detect CMV antigens. The sensitivity and Specificity of each test and all the methods together were evaluated, and SPSS software was used to analysis of data.

From 200 patients, 193 (96.5%) were positive for CMV antibodies with the Specificity of 100 and sensitivity of 97.76%. 120 (60%) and 25 (12.5) samples were positive by nested PCR and Ag assay with the Specificity of 94.49 and 78.12 and sensitivity of 94.49 and 78.12, respectively.

In the case of early diagnosis of the disease, nested PCR diagnose the infection 14 years earlier than Ag assay and was consistently positive, whereas false negative results were frequently observed with the pp65 Ag assay. The sensitivity and specificity of the two methods combined detection for CMV infection were 96.76% and 99.89%. ELISA can be used as a screening reliable detection test for CMV infection in recipient especially when PCR is unavailable.

Combination of ELISA and CMV-PCR methods, provide a more effective method to monitor CMV infection.

Keywords: Cytomegalovirus; Infection; Renal transplantation; Antigenemia assay

Citation: Moghanloo E, Babaei V, Rezaei S, Khanmirzaei L, Bahri TD, et al. (2018) Comparison of ELISA, Antigenemia Assay and Nested PCR Monitoring Techniques for Detection of Cytomegalovirus Infection in Renal Transplantation Patients. J Med Microb Diagn 7: 274. Doi: 10.4172/2161-0703.1000274

Copyright: © 2018 Moghanloo E, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permitsunrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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