alexa Comparison of Follicle-Stimulating Hormone Glycosylation Microheterogenity by Quantitative Negative Mode Nano-Electrospray Mass Spectrometry of Peptide-N-Glycanase-Released Oligosaccharides
ISSN: 2153-0637

Journal of Glycomics & Lipidomics
Open Access

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Research Article

Comparison of Follicle-Stimulating Hormone Glycosylation Microheterogenity by Quantitative Negative Mode Nano-Electrospray Mass Spectrometry of Peptide-N-Glycanase-Released Oligosaccharides

George R. Bousfield1, Vladimir Y. Butnev1, William K. White1, Aaron Smalter Hall2 and David J. Harvey3

1Department of Biological Sciences, Wichita State University, Wichita, KS 67260, USA

2Molecular Graphics and Modeling Laboratory, University of Kansas, Lawrence, KS 66045, USA

3Department of Biochemistry, University of Oxford, Oxford, OX1 3QU, UK

*Corresponding Author:
Bousfield George R
Department of Biological Sciences
Wichita State University, Wichita, KS 67260, USA
E-mail: [email protected]

Received Date: December 15, 2014; Accepted Date: February 18, 2015; Published Date: February 23, 2015

Citation: Bousfield GR, Butnev VY, White WK, Hall AS, Harvey DJ (2015) Comparison of Follicle-Stimulating Hormone Glycosylation Microheterogenity by Quantitative Negative Mode Nano-Electrospray Mass Spectrometry of Peptide-N-Glycanase-Released Oligosaccharides. J Glycomics Lipidomics 4:129. doi: 10.4172/2153-0637.1000129

Copyright: © 2015 Bousfield GR, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Glycans from six highly purified hFSH preparations were released by peptide-N-glycanase digestion and analyzed by negative mode nano-ESI mass spectrometry before and after neuraminidase digestion. Pituitary glycan structures were mainly high-mannose, di-, tri-, and tetra-antennary, and their abundance largely paralleled that reported by other investigators using different approaches. For most of the FSH preparations, the differences in glycosylation appeared to be restricted to relative abundances of the major glycan families, as defined by their neutral core oligosaccharide structures. Qualitative differences between glycan populations were largely relegated to those species that were lowest in abundance. Significant qualitative differences were noted in two cases. Recombinant GH3-hFSH triantennary glycans appeared to have the third antenna exclusively on the mannose6-branch, in contrast to all pituitary and urinary hFSH triantennary glycans, in which this antenna was exclusively attached to the mannose3-branch. The hypo-glycosylated hFSH preparation isolated from purified hLH was decorated with high mannose glycans that accounted for over 40% of the total in this population. As this preparation was found to be consistently 20-fold more active than hFSH24 in FSH receptor-binding assays, it appears that both macroheterogeneity and microheterogeneity in FSH preparations need to be taken into account.

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