Medicinal Chemistry

Comparison of HPLC and UV Spectrophotometric Methods for the Determination of Cefaclor Monohydrate in Pharmaceutical Dosages

Abstract

Alfeen Mohammad A

This paper describes the development and evaluation of a HPLC and UV spectrophotometric methods to quantify Cefaclor Monohydrate in Oral suspensions and Capsules. HPLC analysis were carried out using a C18 Knauer column and a mobile phase composed of Triethylamine:methanol:Acetonitrile:water (2:10:20:68) v/v%, with a flow rate of 1.0 mL/min and UV detection at 265 nm. For the spectrophotometric analysis, water was used as solvent and the wavelength of 264 nm was selected for the detection. Both methods were found to quantify Cefaclor monohydrate in Oral suspensions and Capsules accurately. Therefore HPLC and UV methods presented the most reliable results for the analyses of Oral suspension and Capsules.