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Comparison of Phage-Based Magnetoelastic Biosensors with Taqman- Based Quantitative Real-Time PCR for the Detection of Salmonella typhimurium Directly Grown on Tomato Surfaces | Abstract
ISSN: 2155-6210

Journal of Biosensors & Bioelectronics
Open Access

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Research Article

Comparison of Phage-Based Magnetoelastic Biosensors with Taqman- Based Quantitative Real-Time PCR for the Detection of Salmonella typhimurium Directly Grown on Tomato Surfaces

Mi-Kyung Park1*, Jang Won Park2, Howard C. Wikle, III1 and Bryan A. Chin1

1Materials Research and Education Center, Auburn University, 275 Wilmore Labs, Auburn, Alabama, 36849, USA

2Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390, USA

*Corresponding Author:
Mi-Kyung Park
Materials Research and Education Center
Auburn University, 275 Wilmore Labs
Auburn, Alabama, 36849, USA
Tel: +1 334 844 7443
Fax: +1 334 844 3400
E-mail: [email protected]

Received Date: October 25, 2011; Accepted Date: December 09, 2011; Published Date: December 14, 2011

Citation: Park MK, Park JW, Wikle HC III, Chin BA (2012) Comparison of Phage-Based Magnetoelastic Biosensors with Taqman-Based Quantitative Real-Time PCR for the Detection of Salmonella typhimurium Directly Grown on Tomato Surfaces. J Biosens Bioelectron 3:113. doi: 10.4172/2155-6210.1000113

Copyright: © 2012 Park MK, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

A phage-based magnetoelastic (ME) biosensor method was compared with a TaqMan-based quantitative real- time PCR (Q-PCR) method for the detection of Salmonella typhimurium on tomato surfaces. This ME biosensor method utilizes magnetoelastic resonators coated with E2 filamentous phage to bind with and measure the concentration of S. typhimurium . In this study, standard curves, correlations, and limits of detection (LOD) for the ME biosensor and Q-PCR methods were determined by inoculating tomato surfaces with S. typhimurium suspensions in concentrations ranging from 1 to 8 log CFU/tomato. The LOD for the ME biosensor method and Q-PCR were 3 and 2 log CFU/tomato, respectively. In a direct comparison of the detection methods, S. typhimurium suspensions (3 log CFU/tomato) were inoculated on 65 tomato surfaces, then incubated at 37°C and 100% RH for 24 h. After 24 h, S. typhimurium was positively detected by both methods and the quantified concentrations were nearly the same, (6.35 ± 2.03) and (6.34 ± 0.17) log CFU/tomato respectively for the ME biosensor method and the Q-PCR method, which were significantly greater than the concentration determined by the BGS-plate count method (5.33 ± 0.21). Scanning electron microscopy (SEM) was used to confirm the growth of S. typhimurium on the tomato surfaces and the binding of S. typhimurium on the measurement sensors. This study demonstrated that the ME biosensor method was robust and competitive with Q-PCR for S. typhimurium detection on fresh produce.

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