alexa Comparison of Roche COBAS<sup>andreg;</sup>AmpliPrep/COBAS<sup>andreg;</sup>TaqManHIV-1v2.0 and Abbott m2000sp/m2000rt for the Measurement of HIV-1 Viral Load in Senegal| Abstract
ISSN: 1747-0862

Journal of Molecular and Genetic Medicine
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  • Research Article   
  • J Mol Genet Med 2019, Vol 13(2): 424

Comparison of Roche COBAS®AmpliPrep/COBAS®TaqManHIV-1v2.0 and Abbott m2000sp/m2000rt for the Measurement of HIV-1 Viral Load in Senegal

Faye B1,2,3*, Dieng FB1, Charlebois R4, Sarr H1, Diouf SG1, Diagne MM1, Sembène M5 and Dièye A3
1Laboratory of Molecular Biology, Military hospital of Ouakam (HMO), Dakar, Senegal
2AIDS Program of the Senegalese Armed Forces, Senegal
3Service of Immunology, Department of Pharmacy, Faculty of Medicine, Pharmacy and Odonto-Stomatology of Cheikh Anta Diop University of Dakar, Senegal
4Global Scientific Solutions for Health (GSSHealth), Baltimore, MD, United States
5Genetics Team of Population Management, Department of Animal Biology, Faculties of Science and Technology, Cheikh Anta Diop University of Dakar, Senegal
*Corresponding Author : Dr. Faye B, Laboratory of Molecular Biology, Military Hospital of Ouakam (HMO), Dakar, Senegal, Tel: +221 77 6341070, +221 338601989, Email: [email protected]

Received Date: Apr 23, 2019 / Accepted Date: May 17, 2019 / Published Date: May 24, 2019


Accurate quantification of HIV-1 viral load (VL) is crucial for assessing infection stage and efficacy of antiretroviral therapy (ART). Despite recommendations for measuring VL amongst people living with HIV, the accessibility and availability of this parameter remain low in resource-limited settings, primarily due to the lack of qualified human resources and necessary reagents. Solutions must be found to help developing countries attain the Joint United Nations Program on HIV/AIDS (UNAIDS) 90-90-90 targets for 2020. This study was designed to compare the quantification of HIV-1 VL between two reverse transcriptase real-time PCR techniques: RocheCOBAS® AmpliPrep/ COBAS®TaqMan®HIV-1v2.0 and Abbott m2000sp/m2000rt. To conduct the comparison, 231 samples for VL were assessed. Samples were stratified according to the following VL intervals:<3 Log10; 3 Log10-4 Log10; 4 Log-5 Log10; 5 Log10-6 Log10 and > 6 Log10 copies/ml. The Bland-Altman method and the Bland-Altman plot were used for the comparison of the two techniques. The concordance varies from 92 to 98% depending on the VL interval studied. Our results showed that these two techniques give similar results and that all observed variations are under 0.5 Log10 copies/ml, which is considered a significant variation for treatment failure. This concordance was confirmed by the overall VL comparison obtained using linear regression. The linear regression shows a correlation with R2 = 0.83 and a 95% agreement between the two techniques. Our results show that these techniques are interchangeable and thus, in some contexts, would improve the availability of VL to help achieve the UNAIDS third "90" target set for 2020.

Keywords: Comparison; Abbott; Roche; Viral load; HIV-1; Senegal

Citation: Faye B, Dieng FB, Charlebois R, Sarr H, Diouf SG, et al. (2019) Comparison of Roche COBAS®AmpliPrep/COBAS®TaqManHIV-1v2.0 and Abbott m2000sp/m2000rt for the Measurement of HIV-1 Viral Load in Senegal. J Mol Genet Med 13:424.

Copyright: © 2019, Faye B et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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