Comparison of the Reliability of 17 Celiac Disease Associated Bio-Markers to Reflect Intestinal Damage
- *Corresponding Author:
- Aaron Lerner
AESKU.KIPP Institute. Mikroforum Ring 2
Wendelsheim 55234, Germany
Fax: 49-6734-9622- 2222
E-mail: [email protected]
Received date: October 18, 2016; Accepted date: January 24, 2017; Published date: January 27, 2017
Citation: Lerner A, Jeremias P, Neidhöfer S, Matthias T (2017) Comparison of the Reliability of 17 Celiac Disease Associated Bio-Markers to Reflect Intestinal Damage. J Clin Cell Immunol 8:486. doi: 10.4172/2155-9899.1000486
Copyright: © 2017 Lerner A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
In view of the increasing importance of serological biomarkers for screening and diagnosing celiac disease (CD) and the lack of back-to-back comparison of their differential performance to their reliability to reflect the intestinal damage in children with CD, their performances were evaluated.
95 pediatric CD patients (mean age 8.3), 45 nonspecific abdominal pain children (AP) (mean age 7.3), 99 normal children (NC) (mean age 8.5) were tested with following ELISAs (detecting IgA, IgG or both, IgA and IgG (check)): AESKULISA® Gliadin (AGA), AESKULISA® DGP (DGP), AESKULISA® tTg “New Generation” (Neo-epitope tTg complexed to gliadin=tTg-neo), tTg (for in house research purpose only), AESKULISA® mTg neo-epitope and mTg (RUO). Anti-endomysial antibodies (EMA) were checked via immunofluorescence test. Results were compared to the degree of intestinal injury, using the revised Marsh criteria. Scatter diagrams and regression analysis comparing the 17 antibodies’ activities to the degree of the intestinal damage were performed.
Most of the assays were able to discriminate patients with low and high degree of intestinal damage. Comparing the different correlations of CD associated IgA and IgG antibodies’ isotypes, the tTg-neo IgA (r2=0.6165, p<0.0001) and tTg-neo check (r2=0.6492, p<0.0001) stood out, significantly, as the best indicators for intestinal damage in CD. EMA-IgA, tTg, DGP checks and mTg-neo IgG correlated closely to the mucosal injury.
The highest optical densities (medium 2.94 ± 1.2, p<0.0001) were measured in the tTg-neo IgA ELISA of patients with Marsh 3c.
As a conclusion, it is suggested that tTg-neo IgA/IgG antibodies should be used preferably to closely reflect intestinal damage during screening and diagnosing childhood CD. EMA-IgA, tTg and DGP checks and mTg-neo IgG titers followed the tTg-neo check performance. mTg-neo IgG may present a new serological biomarker for CD.