Comparison of Variants of Carbol Fuchsin & Phenol in Ziehl Neelsen Staining to Detect AFB
|Jaya Chandra T1*, Selvaraj R2 and Sharma YV3|
|1Department of Microbiology, GSL Medical College, Rajahmundry, South India|
|2Associate Professor, Centre for Lab Animal Technology & Research (CLATR), Sathyabama University, Chennai, South India|
|3Professor of Pathology, GSL Medical College, Rajahmundry, South India|
|Corresponding Author :||Jaya Chandra T
Department of Microbiology, GSL Medical College
Rajahmundry, South India
E-mail: [email protected]
|Received August 12, 2013; Accepted November 04, 2013; Published November 11, 2013|
|Citation: Jaya Chandra T, Selvaraj R, Sharma YV (2013) Comparison of Variants of Carbol Fuchsin & Phenol in Ziehl Neelsen Staining to Detect AFB. J Mycobac Dis 3:131. doi:10.4172/2161-1068.1000131|
|Copyright: © 2013 Jaya Chandra T, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
Introduction: Sputum smear microscopy is the mainstay in the rapid diagnosis of pulmonary tuberculosis. Ziehl Neelsen staining is popularly used smear microscopy techniques.
Method: We evaluated the use of 0.1% Basic Fuchsin, 5% phenol containing primary staining reagent (Solution ‘A’) and 0.1% Basic Fuchsin, 7.5% phenol (Solution ‘B’) in the diagnosis of pulmonary tuberculosis. Results are compared with standard.
Results: Out of 196 sputum samples smear positivity were 9.7%, 7.6% and 9.2% respectively with standard
method and Solution ‘A’ and Solution ‘B’.
Conclusion: Even though with some reduction in expenditure, reagents concentrations should not be altered.