alexa Comparison of Variants of Carbol Fuchsin and Phenol in Ziehl Neelsen Staining to Detect AFB | OMICS International | Abstract
ISSN: 2161-1068

Mycobacterial Diseases
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Comparison of Variants of Carbol Fuchsin & Phenol in Ziehl Neelsen Staining to Detect AFB

Jaya Chandra T1*, Selvaraj R2 and Sharma YV3
1Department of Microbiology, GSL Medical College, Rajahmundry, South India
2Associate Professor, Centre for Lab Animal Technology & Research (CLATR), Sathyabama University, Chennai, South India
3Professor of Pathology, GSL Medical College, Rajahmundry, South India
Corresponding Author : Jaya Chandra T
Department of Microbiology, GSL Medical College
Rajahmundry, South India
Tel: 91-9490646100
E-mail: [email protected]
Received August 12, 2013; Accepted November 04, 2013; Published November 11, 2013
Citation: Jaya Chandra T, Selvaraj R, Sharma YV (2013) Comparison of Variants of Carbol Fuchsin & Phenol in Ziehl Neelsen Staining to Detect AFB. J Mycobac Dis 3:131. doi:10.4172/2161-1068.1000131
Copyright: © 2013 Jaya Chandra T, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Introduction: Sputum smear microscopy is the mainstay in the rapid diagnosis of pulmonary tuberculosis. Ziehl Neelsen staining is popularly used smear microscopy techniques.
Method: We evaluated the use of 0.1% Basic Fuchsin, 5% phenol containing primary staining reagent (Solution ‘A’) and 0.1% Basic Fuchsin, 7.5% phenol (Solution ‘B’) in the diagnosis of pulmonary tuberculosis. Results are compared with standard.
Results: Out of 196 sputum samples smear positivity were 9.7%, 7.6% and 9.2% respectively with standard
method and Solution ‘A’ and Solution ‘B’.
Conclusion: Even though with some reduction in expenditure, reagents concentrations should not be altered.

Keywords

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

b[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7