alexa Competitive Enzyme Linked Immuno-Sorbent Assay (c-ELISA
ISSN: 2161-0517

Virology & Mycology
Open Access

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Research Article

Competitive Enzyme Linked Immuno-Sorbent Assay (c-ELISA) Based Sero- Prevalence of Bluetongue Virus (BTV) on Small Ruminants in Selected Areas of Wolyita, Southern Ethiopia

Melese Yilma* and Mesfin Mekonnen

Areka Agricultural research center, Po Box 79, Areka, Ethiopia

Corresponding Author:
Melese Yilma
Areka Agricultural research center
Po Box 79, Areka, Ethiopia
Tel: +251 910 75 51 90
E-mail: [email protected]

Received Date: September 18, 2015; Accepted Date: November 30, 2015; Published Date: Dec 07, 2015

Citation: Yilma M, Mekonnen M (2015) Competitive Enzyme Linked Immuno-Sorbent Assay (c-ELISA) Based Sero-Prevalence of Bluetongue Virus (BTV) on Small Ruminants in Selected Areas of Wolyita, Southern Ethiopia. Virol-mycol 4:148. doi:10.4172/2161-0517.1000148

Copyright: © 2015 Yilma M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

The objective of the present study is to assess the sero-prevalence and associated risk factors for small ruminants’ bluetongue infection in selected agro-ecology of woliyta zone, southern Ethiopia. Serum samples were collected randomly from the accessed small ruminates and screened for detection of BTV-specific immunoglobulin G (IgG) antibodies using a competitive enzyme-linked immunosorbent assay (c-ELISA). A total of 476 serum samples were tested and 196 (41.17%) were positive for bluetongue virus antibodies. A prevalence rate ranging from 26.53% for the midland altitude to 73.47% for the lowland was recorded. To disease associated risk factor age, and location was recorded using multivariate analysis of logistic regression model. Within species goat 114 (58.2%) and sheep 82 (41.8%) sero positive reactors was recorded. This is the first report indicating the presence of bluetongue virus infection in the area.

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