Complex Analysis of 700-Year-Old Skeletal Remains found in an UnusualGraveÃ¢ÂÂCase ReportDaniel Vanek1,11*, Hana Brzobohata2,3, Marcela Silerova11, Zdenek Horak4, Miriam Nyvltova Fisakova5, Michaela Vasinova Galiova6,7, Pavla Zednikova Mala2,8, Vladislava Urbanova11, Miluse Dobisikova9, Michal Beran1 and Petr Brestovansky10
- *Corresponding Author:
- Daniel Vanek
Forensic DNA Service
Janovskeho 18, 170 00 Prague 7
E-mail: [email protected]
Received date: August 04, 2014; Accepted date: January 27, 2015; Published date: February 05, 2015
Citation: Vanek D, Brzobohata H, Silerova M, Horak Z, Nyvltova Fisakova, Vasinova Galiova M, et al. (2015) Complex Analysis of 700-Year-Old Skeletal Remains found in an Unusual Grave–Case Report. Anthropol 2:138. doi:10.4172/2332-0915.1000138
Copyright: © 2015 Vanek D, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Aim: The present study was designed to analyze the 700-year-old human remains from an unusual grave using a combined approach that consisted of anthropological, archaeogenetic, genealogical, mass spectrometry, 3-dimensional (3D) modeling and facial reconstruction methods to confirm or reject several hypotheses about the skeletal remains. Methods: DNA was extracted from the skeleton and amplified using autosomal and Y-chromosome human identification short tandem repeat (STR) kits that were designed for forensic use, and sequence data were obtained from hyper variable region I (HVRI) mtDNA sequencing. Elemental mapping and quantification of investigated elements were performed using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The Computed Tomography (CT) images of the skull were created in a transversal plane, and the scans were used to create 3D geometric models of the skull. A plastic physical model (a cast) of the skull was produced by rapid prototyping technology, and the model was used for sculptural facial approximation of the studied individual. Results: The Y-chromosome haplogroup of the sample was determined to be E1b1b, and the assigned mtDNA haplogroup was H. LA-ICP-MS and geochemical analysis revealed that the individual consumed plants and meats, except pork. Anthropological examination estimated the age of the individual to be between 45-55 years, and we did not find any traces of disablement or physical anomalies. Interestingly, we were able to produce a facial reconstruction according to the skull. Conclusion: Applying a multidisciplinary approach to the examination of the 14th century material enabled us to retrieve new types of information that helped us to interpret the excavated skeletal remains.