Compound Isolation and Purification by Chromatographic Method of Stem Bark of Anisoptera scaphula (Roxb.).
Anisoptera scaphula (Roxb.) belonging to the family Dipterocarpaceae, has been studied for isolation of its secondary metabolites and evaluation of biological activities of the extractives, with special emphasis on TLC chromatographic data. Identification and isolation of the active constituents from traditionally used phytotherapy can ensure the health care of the poor people. In addition, herbal drugs could be scientifically modified for better pharmacological activity and to establish safe and effective drugs and the rationality of the present study lies in meeting the challenge of developing herbal medicines, which needs a systematic research on indigenous medicinal plants for the welfare of the humanity. This study will help to elucidate the hidden potential compounds which may be helpful for human being. The stem bark of A. scaphula was extracted with organic solvents and the extracts were fractionated with standard chromatographic techniques. A total of five compounds were isolated from the investigated plant. Chromatographic fractionation and purification of the pet-ether extract yielded a triterpene (AS-1), which was purified and identified as stigmasterol. The ethyl acetate extract yielded a total of five compounds and Characterization of these isolated compounds by 1H NMR spectrum is underway. Therefore plant materials, especially the traditionally used medicinal plants can be potential sources of chemically interesting and biologically important drug candidate.