alexa Comprehensive Analysis of Phytopharmaceutical Formulations┬ľAn Emphasis on Two-Dimensional Liquid Chromatography
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
Open Access

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Research Article

Comprehensive Analysis of Phytopharmaceutical Formulations┬ľAn Emphasis on Two-Dimensional Liquid Chromatography

M Luísa S Silva*

Centre of Chemical Research, Autonomous University of Hidalgo State, Carr. Pachuca-Tulancingo km 4.5, 42184 Pachuca, Hidalgo, México

*Corresponding Author:
Maria Luísa S. Silva
Centro de Investigaciones Químicas
Universidad Autónoma del Estado de Hidalgo
Carr. Pachuca-Tulancingo km 4.5
42184 Pachuca, Hidalgo, México
Tel: (+52) 771 7172000 ext 2217
Fax: (+52) 771 7172000 ext 6502
E-mail: [email protected]

Received date: October 31, 2015; Accepted date: January 08, 2015; Published date: January 23, 2015

Citation: Silva MLS (2015) Comprehensive Analysis of Phytopharmaceutical Formulations – An Emphasis on Two-Dimensional Liquid Chromatography. J Chromatogr Sep Tech 6:262. doi:10.4172/2157-7064.1000262

Copyright: © 2015 Silva MLS. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



In spite of the efforts in increasing the analytical control on herbal medicines, difficulties in the standardization of their components and the absence of a thorough characterization of the chemical composition still persist and constitute obstacles to the safe therapeutic use of these substances. The public consider these products inoffensive and uses them intensively or extensively, including specific groups of population like children and elder people. Thus, it is important to know as much as possible the chemical composition of these phytopharmaceutical products and quantify their pharmacologically active components. The analysis of plant extracts is a difficult task, as they are usually very complex mixtures. The separation power of traditional one-dimensional methodologies is generally inadequate, so two-dimensional separation is required to provide enhanced separation, since it enables to achieve higher overall peak capacities. Furthermore, comprehensive 2D analysis can provide a fingerprinting analysis of samples, allowing the detection of differences in the presence and/or concentration of compounds in complex mixtures.


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