Comprehensive Study of Oral Squamous Cell Carcinoma Patients Using Blood Samples and Gene Expression Profiles
|Nobuo Kondoh1*, Eiji Takayama1, Masako Kamiya1, Harumi Kawaki1, Masayuki Motohashi2, Yasunori Muramatsu2, Michio Shikimori2, Kenji Mitsudo3and Iwai Tohnai3|
|1Department of Oral Biochemistry, Asahi University School of Dentistry, Mizuho-shi, Hozumi 1851Gifu 501-0296, Japan|
|2Department of Oral Surgery, Asahi University School of Dentistry, Mizuho-shi, Hozumi 1851Gifu 501-0296, Japan|
|3Department of Oral and Maxillofacial Surgery, Yokohama City University Graduate School of Medicine, Yokohama-shi, Kanagawa 236-0004, Japan|
|Corresponding Author :||Nobuo Kondoh
Department of Oral Biochemistry
Asahi University School of Dentistry
Mizuho-shi, Hozumi 1851
Gifu 501-0296, Japan
Tel: 81 58 1416
Fax: 81 58 329 1417
E-mail: [email protected]
|Received November 01, 2012; Accepted November 24, 2012; Published November 26, 2012|
|Citation: Kondoh N, Takayama E, Kamiya M, Kawaki H, Motohashi M, et al. (2012) Comprehensive Study of Oral Squamous Cell Carcinoma Patients Using Blood Samples and Gene Expression Profiles. J Cancer Sci Ther S18:001. doi: 10.4172/1948-5956.S18-001|
|Copyright: © 2012 Kondoh N, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
|Related article at
Pubmed Scholar Google
Oral squamous cell carcinoma (OSCC) is an aggressive malignancy which shows a variable degree of malignant behavior. To identify molecular signatures and establish a new diagnostic model for oral malignancies, we have identified marker genes representing pre-malignant and malignant phenotypes of oral mucosal lesions. The expression of marker genes was examined by quantitative reverse transcription-PCR. Then, we created discriminatory predictor models using Fisher’s linear discriminant analysis and leave-one-out cross validation. These models were applicable for the diagnoses of pre-malignant leukoplakias (LPs), and of invasion status for advanced OSCCs.
The clinical course of various cancers is also influenced by host immune response. Our preliminary data using flow cytometric analysis demonstrate that the percentage of CD4+CD57+ T cells in peripheral blood lymphocyte was higher in the high grade OSCCs than that in the low grade ones. Furthermore, lipopolysaccharides (LPS)-induced ex-vivo production of Interferon (IFN)-γ from peripheral blood cells was highest in stage I patients and gradually decreased during the course of OSCC progression up to stage III. These decreased levels in the early stages were inversely correlated with tumor size.
In this review, we propose that the usage of the immunological status of OSCC patients combined with the molecular signatures of tumor tissues could provide valuable indices for diagnosis of oral malignancies.