Concerted Evolution of the Replication-Dependent Histone Gene Family in Drosophila immigransKakubayashi N1,2, Fujita E1, Morikawa M1, Ohashi S1 and Matsuo Y1-4*
- *Corresponding Author:
- Matsuo Y
Graduate School of Science and Technology
Tokushima University, Minamijosanjima-cho 2-1
Tokushima, 770-8506, Japan
E-mail: [email protected]
Received date: December 02, 2016; Accepted date: December 19, 2016; Published date: January 05, 2017
Citation: Kakubayashi N, Fujita E, Morikawa M, Ohashi S, Matsuo Y (2017) Concerted Evolution of the Replication-Dependent Histone Gene Family in Drosophila immigrans. J Data Mining Genomics Proteomics 8:210. doi: 10.4172/2153-0602.1000210
Copyright: © 2017 Kakubayashi N, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The replication-dependent histone genes in Drosophila immigrans were analyzed for elucidating the evolutionary mechanism of the histone multigene family. A region of approximately 3.9 kb containing H2A-H2B-H1 genes was cloned. Six independent clones were sequenced and analyzed for nucleotide variability. The average nucleotide sequence identity in the region among repetitive copies was more than 99%, indicating that the histone multigene family in D. immigrans has evolved in a concerted fashion and with a similar level as in D. melanogaster. Amino acid variants were found at a low frequency. Analysis of the GC content at the 3rd codon position of histone genes revealed that a change in GC content, i.e., a decrease, observed in D. hydei and D. americana has occurred after the divergence of an ancestor of these two species from D. immigrans.