Concomitant Effect of Chlorpyrifos and Intranasal Endotoxin Administration on Apoptosis Related Protein Expression in Lung of Mice
Received Date: Oct 24, 2012 / Accepted Date: Dec 28, 2012 / Published Date: Dec 31, 2012
Pesticides and bacterial endotoxin have been implicated in variety of respiratory dysfunctions. In present study, mice were administered chlorpyrifos at 3 mg/Kg BW/day for 30 days (by oral gavage) and/or challenged with bacterial endotoxin at 80 μg/mice by intranasal route to investigate expression of apoptosis related proteins such as p53, Bax and caspase-3 in lung. The results of immunohistochemistry of lung showed significant increase in number of p53 expressing cells in chlorpyrifos treated mice as compared to control while endotoxin and chlorpyrifos+endotoxin treated mice showed increased number of p53 expressing cells as compared to control and chlorpyrifos treated mice. Chlorpyrifos or endotoxin administration resulted in increased expression of Bax and Caspase-3 while chlorpyrifos treated and endotoxin challenged mice showed synergistic effect on expression of these proteins. From these results, it is concluded that chlorpyrifos and/or endotoxin increased critical apoptosis related proteins such as p53, Bax and Caspase-3 in lung of mice, which may play pivotal role in pathophysiology of pulmonary dysfunctions resulting from exposure to pesticides and endotoxin.
Keywords: Apoptosis; Chlorpyrifos; Endotoxin; Mice; Pesticide
Citation: Chougule AA, Brar RS, Banga HS, Singh ND, Goyal A, et al. (2013) Concomitant Effect of Chlorpyrifos and Intranasal Endotoxin Administration on Apoptosis Related Protein Expression in Lung of Mice. J Environ Anal Toxicol 3:164. Doi: 10.4172/2161-0525.1000164
Copyright: © 2013 Chougule AA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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