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Concordance Study between the AmpFlSTRandreg; SGM Plusandtrade; and PowerPlexandtrade; 16 System Human Identification Kits in Bangladeshi Population | OMICS International | Abstract
ISSN: 2157-7145

Journal of Forensic Research
Open Access

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Research Article

Concordance Study between the AmpFlSTR® SGM Plus™ and PowerPlex™ 16 System Human Identification Kits in Bangladeshi Population

Shafiul Alam2, Ahmad Ferdous2, Mohammad Eunus Ali2, Tania Hossain2, Mahamud Hasan2 and Sharif Akhteruzzaman1*

1Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka -1000, Bangladesh

2National Forensic DNA Profiling Laboratory, Nuclear Medicine Building (10th Floor), Dhaka Medical College Campus, Dhaka- 1000, Bangladesh

*Corresponding Author:
Sharif Akhteruzzaman
PhD., Professor, Department of Biochemistry and Molecular Biology
University of Dhaka, Dhaka-1000, Bangladesh
Tel: +88-01714111267
Fax: +8802-8615583
E-mail: [email protected]

Received date: December 08, 2010; Accepted date: February 10, 2011; Published date: February 14, 2011

Citation: Alam S, Ferdous A, Ali ME, Hossain T, Hasan M, et al. (2011) Concordance study between the AmpFlSTR® SGM plus™ and PowerPlex® 16 system human identification kits in Bangladeshi population. J Forensic Res 2:116. doi:10.4172/2157-7145.1000116

Copyright: © 2011 Alam S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

In this study, we are reporting the concordance study between profiles from AmpFlSTR ® SGM plus™ and that of PowerPlex ® 16 system kits in Bangladeshi Bangali population. DNA samples from 234 unrelated Bangladeshi Bangali individuals were evaluated employing both the SGM plus™ and PowerPlex ® 16 system human identification kits. Complete consistency was observed in 233 out of 234 profiles which represents substantial degree of concordance amounting 99.57% between these two kits. Only one discrepancy encompassing the dropout of allele 12 at D16S539 loci in PowerPlex ® 16 system was observed. Sequence analysis revealed two mutational events; one at 16 bp distal and another next to the 3′ end of the PowerPlex 16 reverse primer of D16S539 locus. This study has shown the significance of using of alternative set of primer to address the false homozygosity issue as well as of bringing attention regarding the fact of discrepant typing from different sets of primers to the scientific community during database handling and identity or kinship investigations.

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