alexa Connexin-36 Knock-Out Mice have Increased Threshold for
ISSN: 2167-0501

Biochemistry & Pharmacology: Open Access
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Research Article

Connexin-36 Knock-Out Mice have Increased Threshold for Kindled Seizures: Role of GABA Inhibition

Samuel I Shin, Dallin J Andersen, Micah Hansen D, Jordan T Yorgason, Nathan D Schilaty, David D Busath and Scott C Steffensen*
Department of Psychology and Neuroscience Center, Brigham Young University, Provo, UT 84602, USA
Corresponding Author : Scott C Steffensen
Department of Psychology (SWKT 1050)
Brigham Young University, Provo, UT 84602, USA
Tel: (801) 422-9499
Fax: (801) 422-0602
E-mail: [email protected]
Received March 02, 2013; Accepted April 15, 2013; Published April 18, 2013
Citation: Shin SI, Andersen DJ, Hansen DM, Yorgason JT, Schilaty ND, et al. (2013) Connexin-36 Knock-Out Mice have Increased Threshold for Kindled Seizures: Role of GABA Inhibition. Biochem Pharmacol (Los Angel) S1:006. doi:10.4172/2167-0501.S1-006
Copyright: © 2013 Shin SI, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 

Abstract

Blockade of gap junctions (GJs) has been shown to reduce seizures in different epilepsy models. Gap junction mediated, electrically-coupled neuronal networks have been implicated in neuronal synchronization, which is the hallmark of seizure activity. To further understand the role of GJs in seizures, in particular hippocampal seizures, we evaluated electrophysiological responses in the dentate gyrus subfield of the hippocampus, including GABA-mediated recurrent inhibition, seizuregenic stimulation, and seizure activity in response to perforant path kindling in Cx36 knockout (KO) mice compared to wild-type (WT) controls. In anesthetized mice, Cx36 KO mice were characterized by enhanced GABA-mediated recurrent inhibition. Stimulation of the perforant path at 10 Hz for 10 sec (i.e., 100 pulses) markedly enhanced population spike (PS) amplitudes and reduced paired-pulse GABA-mediated inhibition during the stimulation, induced an after discharge for approximately 10 sec at 7-10 sec into the stimulation, and suppressed PS amplitudes postictally for 5-10 min in both KO and WT mice. Repeated epochs of 10 Hz for 10 sec stimulation of the perforant path at 20 min intervals resulted in progressive and persistent disinhibition of paired-pulse responses in WT, but not KO mice. In freely-behaving seizure studies, stimulation of the perforant path (10 Hz for 10 sec) once/day resulted in progressive Stage I-IV seizures in both WT and KO mice. Once Stage IV was achieved, another Stage IV seizure could be elicited each day with the same behavioral response (i.e., Stage V). The threshold for kindled seizures was significantly greater in KO mice compared to WT controls for most stages of seizures. The Cx36 antagonist mefloquine (MFQ) and the typical anticonvulsant pentobarbital reduced Stage IV seizures in both WT and KO mice. Knock-out mice were more sensitive to the anti-epileptic effects of the typical anti-convulsant pentobarbital (20 mg/ kg). Taken together, these findings support the emerging view that reduction in GJ-mediated GABA electrical coupling reduces seizures, perhaps through enhancement of GABAergic feedback inhibition, which results from the uncoupling of the GABA recurrent interneurons from the resistive load that is inherent in their electrical connectivity.

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