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ISSN: 2157-7633

Journal of Stem Cell Research & Therapy
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Research Article

Consequences of Simulated Microgravity in Neural Stem Cells: Biological Effects and Metabolic Response

Marianna Silvano1,#, Evelina Miele1,2,#, Mariacristina Valerio3,#, Luca Casadei3, Federica Begalli1, Antonio Francesco Campese1, Zein Mersini Besharat1, Vincenzo Alfano1, Luana Abballe1, Giuseppina Catanzaro4, Maddalena Napolitano1, Alessandra Vacca4, Isabella Screpanti1,2, Cesare Manetti3, Elisabetta Ferretti4,*,$ and Agnese Po1,$

1Department of Molecular Medicine, Sapienza University, Rome 00161, Italy

2Center for Life Nano Science, Sapienza, Istituto Italiano di Tecnologia, Rome 00161, Italy

3Department of Chemistry, Sapienza University, 00185 Rome, Italy

4Department of Experimental Medicine, Sapienza University, Rome 00161, Italy

#These authors contributed equally

$co-senior authors

*Corresponding Author:
Elisabetta Ferretti, MD PhD
Department of Experimental Medicine - Sapienza University
Rome, Italy Viale Regina Elena, 291 - 00161 Rome, Italy
Tel: +39 0649255135
E-mail: [email protected]

Received date: May 29, 2015; Accepted date: June 27, 2015; Published date: June 30, 2015

Citation: Silvano M, Miele E, Valerio MC, Casadei L, Begalli F et al. (2015) Consequences of Simulated Microgravity in Neural Stem Cells: Biological Effects and Metabolic Response. J Stem Cell Res Ther 5:289. doi:10.4172/2157-7633.1000289

Copyright: © 2015 Silvano, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Objective: Microgravity was often shown to cause cell damage and impair cell cycle in a variety of biological systems. Since the effects on the neural system were poorly investigated, we aimed to gain insight into how biological processes such as cell cycle, cell damage, stemness features and metabolic status are involved in neural stem cells (NSC) when they experience simulated microgravity. We also wished to investigate whether these modulations were transient or permanent once cells were returned to normal gravity.
Methods: NSC were isolated from mouse cerebella and cultured in the Rotary Cell Culture System (RCCS) to model microgravity. We analyzed cell cycle, stress and apoptotic response. We also performed a 1H NMR-based metabolomic analysis and evaluation of stemness features of NSC in simulated microgravity and once in the returned to normogravity cell culture.
Results: Biological processes and metabolic status were modulated by simulated microgravity. Cells were arrested in S-phase together with enhanced apoptosis. Metabolic changes occurred in NSC after simulated microgravity. Interestingly, these modulations were transient. Indeed, stemness features and metabolic footprint returned to basal levels after few days of culture in normal conditions. Moreover NSC clonogenic ability was not impaired.
Conclusions: Our data suggest that simulated microgravity impacts on NSC biological processes, including cell cycle and apoptosis. However, NSC does not suffer from permanent damage.


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