alexa Constitutive GFP-UTF1 Expression Interferes with ES and EC Cell Differentiation | OMICS International | Abstract
ISSN: 2157-7633

Journal of Stem Cell Research & Therapy
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Constitutive GFP-UTF1 Expression Interferes with ES and EC Cell Differentiation

Rajkumar P. Thummer, Loes J. Drenth-Diephuis and Bart J.L. Eggen*

Department of Neuroscience, Section Medical Physiology, University of Groningen, University Medical Center Groningen, A. Deusinglaan 1, 9713 AV Groningen, The Netherlands

*Corresponding Author:
Bart J.L. Eggen
Department of Neuroscience
Section Medical Physiology, University of Groningen
University Medical Center Groningen
A. Deusinglaan 1, 9713 AV Groningen
The Netherlands
Tel: +31-50-3632136
Fax: +31-50-3632751
E-mail: [email protected]

Received date July 24, 2012; Accepted date September 11, 2012; Published date September 14, 2012

Citation: Thummer RP, Drenth-Diephuis LJ, Eggen BJL (2012) Constitutive GFPUTF1 Expression Interferes with ES and EC Cell Differentiation. J Stem Cell Res Ther 2:127. doi:10.4172/2157-7633.1000127

Copyright: © 2012 Thummer RP, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Embryonic stem cells have the ability to self-renew and can differentiate into all cell types of the three embryonic germ lineages. The undifferentiated embryonic cell Transcription Factor 1 (UTF1) gene is highly expressed in ES cells and we previously reported that UTF1 is tightly associated with chromatin and is required for differentiation of pluripotent mouseembryonic stem (ES) and embryonic carcinoma (EC) cells. In this study, we generated ES and EC cell lines constitutively expressing GFP-UTF1 to further investigate its role in differentiation. ES and EC cells constitutively expressing GFP-UTF1were suppressed in their proliferation and were still dependent on LIF for self-renewal. Embryoid body (EB) differentiation of GFP-UTF1 overexpressing ES cells showed both normal differentiation as well as a delayed or incomplete differentiation of a subset of cells. GFP-UTF1 was persistently expressed in undifferentiated cells whereas GFP-UTF1 expression was not detected in differentiated cells. Where GFP-UTF1 expressing ES cells differentiated normally in response to DMSO, EC cell differentiation was completely blocked. When ES and EC cells expressing GFP-UTF1 were treated with RA, differentiation markers were induced and endogenous UTF1 and GFP-UTF1 protein levels decreased. However, GFP-UTF1 and UTF1 (in ES cells) mRNA was still detected indicating that degradation of (GFP-)UTF1 protein preceded down regulation of (GFP-) UTF1 mRNA, suggesting that RA induced UTF1 degradation. Summarizing these data indicate that similar to UTF1 depletion, overexpression of GFP-UTF1 interfered with ES and EC cells differentiation.

Keywords

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

bornova escort

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7