alexa Controlling Shear Stress in a Suspension Culture using Couette Flow for Efficient Proliferation of HEK 293 Cells
ISSN: 2476-2296

Fluid Mechanics: Open Access
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Research Article

Controlling Shear Stress in a Suspension Culture using Couette Flow for Efficient Proliferation of HEK 293 Cells

Nur Khatijah Mohd Zin1,2, Katsuhisa Sakaguchi1,2*, Yuji Haraguchi2, Azuma Takahashi1, Sara Suzuki1, Takanobu Yagi1, Tatsuya Shimizu2 and Mitsuo Umezu1

1School of Advance Science and Engineering, TWIns, Waseda University, Wakamatsu-cho, Shinjuku-ku, Tokyo, Japan

2Institute of Advanced Biomedical Engineering and Science, TWIns, Tokyo Women’s Medical University, Tokyo, Japan

Corresponding Author:
Sakaguchi K
School of Advance Science and Engineering, TWIns, Waseda University
2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan
Tel: +81-3-5367-9945
Fax: +81-3-3359-6046
E-mail: [email protected]

Received Date: May 12, 2016; Accepted Date: May 31, 2016; Published Date: June 06, 2016

Citation: Sakaguchi K, Zin NKM, Haraguchi Y, Takahashi A, Suzuki S, et al. (2016) Controlling Shear Stress in a Suspension Culture using Couette Flow for Efficient Proliferation of HEK 293 Cells. Fluid Mech Open Acc 3:124. doi:10.4172/fmoa.1000124

Copyright: © 2016 Nur Khatijah Mohd Zin, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

The suspension culture system is an increasingly popular method of culturing cells not only because of its up scaling ability, but also the non-enzymatic procurement of cells that is crucial for biomedical research, especially in the fields of pharmacology and regenerative medicine. Hypothetically, by controlling and reducing the shear stress applied to cells in a culture system, the higher viability and proliferation rates. In this study, we analyzed HEK 293 cells cultured with a commercially available spinner flask and our newly developed spinner flask which utilizes the theory of Couette flow for controlling shear stress. Fluid analysis and metabolic analysis of the cultured cells were measured at three different rotational speeds, 40, 50 and 60 rpm. It was apparent that 50 rpm was by far the best speed to proliferate the cells. A further viability test was also done in order to validate our hypothesis. Furthermore, by using the metabolic analysis results, it was observed that in the controlled stress system, the consumption of glucose doubled and lactate production was significantly higher compared to cells that were maintained in the conventional suspension method. Thus, Couette flow based suspension culture system will be a major contributor to the future biomedical and pharmacological field.

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