alexa Convulsive Seizure Protection after Hippocampal Transplantation of Mesenchymal Cells from Adipose Tissue in Mice | OMICS International | Abstract
ISSN: 2157-7633

Journal of Stem Cell Research & Therapy
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Research Article

Convulsive Seizure Protection after Hippocampal Transplantation of Mesenchymal Cells from Adipose Tissue in Mice

Tamura BP1#, Almeida DC2#, Felizardo RJ2, Olanda GC1, Bocca LF1, Pinhal NS1, Alves-de-Moraes LBC1, Covolan L1, Câmara NOS2,3 and Longo BM1*
1Laboratory of Neurophysiology, Department of Neurophysiology, Federal University of São Paulo - UNIFESP – São Paulo, Brazil
2Laboratory of Clinical and Experimental Immunology, Department of Medicine – Nephrology, UNIFESP – São Paulo, Brazil
3Laboratory of Transplant Immunology, Department of Immunology, University of São Paulo – USP, Brazil
#Authors contributed equally to this work
Corresponding Author : Beatriz Monteiro Longo
Neurofisiologia, Depto. Fisiologia - UNIFESP- SP, R. Botucatu, 862 5 andar
V. Clementino – São Paulo- SP, Brazil
Tel: (5511) 5579-2033; 5576-4513
E-mail: [email protected], [email protected]
Received March 01, 2014; Accepted April 16, 2014; Published April 18, 2014
Citation: Tamura BP, Almeida DC, Felizardo RJ, Olanda GC, Bocca LF, et al. (2014) Convulsive Seizure Protection after Hippocampal Transplantation of Mesenchymal Cells from Adipose Tissue in Mice. J Stem Cell Res Ther 4: 196. doi: 10.4172/2157-7633.1000196
Copyright: © 2014 Tamura BP, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

A considerable number of epileptic patients had become resistant to antiepileptic drugs, justifying the need for development of new therapeutic strategies to treat epilepsy. The use of mesenchymal stem cells is an innovative and accessible strategy for the treatment of neuronal disorders, due to their involvement in immunoregulatory mechanisms, trophic and anti-apoptotic action. Objective: Based on this evidence, we evaluated the protective effect of mesenchymal cells from adipose tissue (MCAT) by behavioral and inflammatory responses against convulsive seizure induced by maximum electroconvulsive shock (MES). Methods: MCAT cells were transplanted into the hippocampus of adult male mice, and ten days after the transplantation MES stimulation was applied to induce a generalized tonic-clonic seizure. To evaluate the anticonvulsant activity of MCAT cells, we evaluated the parameters involved with: protection and reduction in the duration of tonic phase, reduction in the mortality rate, and alteration in the hippocampal gene expression of IL-1beta, IL-6, IL-4, IL-10, caspase-1, iNOS and TNFα. Results: MCAT cells transplanted into the hippocampus altered the convulsive threshold, showed anticonvulsant effect by protecting from tonic seizures and mortality and reduced the hippocampal expression of transcripts related to inflammatory response such as IL-1beta, IL-6, caspase-1 and iNOS and increased the level of anti-inflammatory interleukin IL-4. Conclusion: The anticonvulsant effects of the MCAT cells on acute convulsive seizure may be related to inhibitory factors and immunomodulatory mechanisms assigned to mesenchymal cells in the hippocampus. These anticonvulsants mechanisms of MCAT cells bring strong therapeutic implications for the control of epileptic seizures.

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