Corneal Spheres derived from Human Embryonic and Human Pluripotent Parthenogenetic Stem CellsAlina Ostrowska1*, Jackie Cochran2, Larissa Agapova1, Amber Buz’Zard1, Nikolay Turovets1#, Jeremy Hammond2, Irina Turovets1, Subramanian Krishnakumar3, Andrey Semechkin1, Judith Kelleher-Andersson5, Jeffrey Janus1 and Marie Csete4
- Corresponding Author:
- Alina Ostrowska PhD
International Stem Cell Corporation
5950 Priestly Drive, Carlsbad CA 92008
E-mail: [email protected]
Received Date: November 02, 2011; Accepted Date: December 03, 2011; Published Date: December 05, 2011
Citation: Ostrowska A, Cochran J, Agapova L, Buz’Zard A, Turovets N, et al. (2011) Corneal Spheres derived from Human Embryonic and Human Pluripotent Parthenogenetic Stem Cells. J Stem Cell Res Ther S2:006. doi:10.4172/2157-7633.S2-006
Copyright: © 2011 Ostrowska A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Corneal blindness is common. Cornea transplants are the most commonly performed organ transplants, but the need for corneal grafts worldwide far outweighs the supply of healthy donor corneas. Here we describe a differentia - tion protocol that yields corneal orbs from human embryonic stem cells (hESC) as well as from human pluripotent parthenogenetic stem cells (hpSC), and therefore can be manufactured free of transmissible pathogens. Cornea and other tissues generated from parthenogenetic stem cells that are homozygous at HLA loci have a distinct im - munologic advantage over fully allogeneic grafts, and this report is the first to describe multilayered cornea gener - ated from hpSC. The differentiated corneal product is layered and anatomically similar to normal human cornea, expresses appropriate corneal markers at the mRNA and protein (and secreted protein) levels, and is permeable to topical ophthalmic drugs. This 3D stem cell-derived cornea is a foundational step in development of appropriately organized, functional corneal grafts from hESC and hpSC for use in in vitro assays as well as regenerative therapies.