Correlation of Changes of Cho–K1 Cells Metabolism to Changes in Protein Expression in Camp DifferentiationSpera R, Vasile F, Pechkova E and Nicolini C*
Nanoworld Institute , Fondazione EL.B.A.Nicolini, Pradalunga, Bergamo, and Laboratory of Nanobiotechnology and Biophysics, Genoa University, Genova, Italy
- *Corresponding Author:
- Claudio Nicolini
President Nanoworld Institute
Largo Redaelli 7, Pradalunga, Bergamo, Italy
E-mail: [email protected]
Received date: February 03, 2013; Accepted date: March 04, 2013; Published date: March 07, 2013
Citation: Spera R, Vasile F, Pechkova E, Nicolini C (2013) Correlation of Changes of Cho–K1 Cells Metabolism to Changes in Protein Expression in Camp Differentiation. Altern Integr Med 2: 105. doi:10.4172/2327-5162.1000105
Copyright: © 2013 Spera R, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A correlated investigation of cell metabolism and protein expression of Chinese Hamster Ovary cells (CHO-K1) under different growth conditions was performed by 1H nuclear magnetic resonance (1H-NMR), mass spectrometry (MS) and biochemical assays. CHO fibroblasts have shown different metabolic products when grown in different media and when differentiation is stimulated by adenosine 3’, 5’ cyclic monophosphate (cAMP). In particular, while addition of Fetal Calf Serum causes an increase in the glucose metabolism correlated to changes in lipid composition of the membrane observed by 1 H-NMR, differentiation induced by cAMP causes biochemical differences in glucose and lipidic metabolism uniquely correlated both to the specific changes in the composition of nuclear proteins, revealed by mass spectrometry, and to the differences in metabolism, determined by NMR.