alexa Cross-Platform Urine Metabolomics of Experimental Hyperglycemia in Type 2 Diabetes | OMICS International | Abstract
ISSN: 2155-6156

Journal of Diabetes & Metabolism
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Cross-Platform Urine Metabolomics of Experimental Hyperglycemia in Type 2 Diabetes

Liesbet Temmerman2,3#, Alysha M De Livera1#, Jairus B Bowne1, John R Sheedy1, Damien L Callahan2, Amsha Nahid1, David P De Souza1, Liliane Schoofs3, Dedreia L Tull1, Malcolm J McConville1, Ute Roessner2,6* and John M Wentworth4,5

1Metabolomics Australia, Bio21 Institute, 30 Flemington Road, 3052 Victoria, Australia

2Metabolomics Australia, School of Botany, The University of Melbourne, 3010 Victoria, Australia

3Functional Genomics and Proteomics, K.U.Leuven, Naamsestraat 59, 3000 Leuven, Belgium

4Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, 3052 Victoria, Australia

5Centre for Obesity Research and Education, Monash University, 3004 Victoria, Australia

6Australian Centre for Plant Functional Genomics, School of Botany, The University of Melbourne, 3010 Victoria, Australia

#Equal Contribution

*Corresponding Author:
Ute Roessner
School of Botany
The University of Melbourne
3010 Victoria, Australia
Tel: +61 3 90353635
E-mail: [email protected]

Received date November 26, 2011; Accepted date January 18, 2012; Published date January 23, 2012

Citation: Temmerman L, De Livera AM, Bowne JB, Sheedy JR, Callahan DL, et al. (2012) Cross-Platform Urine Metabolomics of Experimental Hyperglycemia in Type 2 Diabetes. J Diabetes Metab S6:002. doi:10.4172/2155-6156.S6-002

Copyright: © 2012 Temmerman L, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Hyperglycemia causes diabetic nephropathy, a condition for which there are no specific diagnostic markers that predict progression to renal failure. Here we describe a multiplatform metabolomic analysis of urine from individuals with type 2 diabetes, collected before and immediately following experimental hyperglycemia. We used targeted nuclear magnetic resonance spectroscopy (NMR), liquid chromatography - mass spectrometry (LC-MS) and gas chromatography - MS (GC-MS) to identify markers of hyperglycemia. Following optimization of data normalisation and statistical analysis, we identified a reproducible NMR and LC-MS based urine signature of hyperglycemia. Significant increases of alanine, alloisoleucine, isoleucine, leucine, N-isovaleroylglycine, valine, choline, lactate and taurine and decreases of arginine, gamma-aminobutyric acid, hippurate, suberate and N-acetylglutamate were observed. GC-MS analysis identified a number of metabolites differentially present in post-glucose versus baseline urine, but these could not be identified using current metabolite libraries. This analysis is an important first step towards identifying biomarkers of early-stage diabetic nephropathy.


Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals


[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7