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ISSN: 2375-4508

Journal of Fertilization: In Vitro - IVF-Worldwide, Reproductive Medicine, Genetics & Stem Cell Biology
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Research Article

Cryorecovery of Mouse Sperm by Different IVF Methods Using MBCD and GSH

Ming-Wen Li, Olivia C Glass, Jasmin Zarrabi, Lisa N. Baker and Kent Lloyd KC*
Mouse Biology Program, University of California, Davis, CA 95618, United States
*Corresponding Author : K. C. Kent Lloyd
Mouse Biology Program, University of California
2795 Second Street, Suite 400, Davis
CA 95618, United States
Tel: (530) 754-6687
Fax: (530) 757-3277
E-mail: [email protected]
Received February 29, 2016; Accepted March 11, 2016; Published March 18, 2016
Citation: Li MW, Glass OC, Zarrabi J, Baker LN, Kent Lloyd KC (2016) Cryorecovery of Mouse Sperm by Different IVF Methods Using MBCD and GSH. J Fertil In Vitro IVF Worldw Reprod Med Genet Stem Cell Biol 4:175. doi:10.4172/2375-4508.1000175
Copyright: © 2016 Li MW, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Different protocols incorporating methyl-β-cyclodextrin (MBCD) and reduced glutathione (GSH) have been reported to improve IVF recovery of cryopreserved mouse sperm on a C57BL/6 (J and N) genetic background. However, it is not clear which IVF protocol is most appropriate when using the various methods to cryorecover sperm with different sperm quality and sample volumes. Therefore, in the present study we correlated sperm motility with fertilization rate and compared the efficiency of different IVF methods using various sperm samples so as to establish general guidelines for mouse sperm cryorecovery by IVF. High linear correlation between sperm fertilization rate and progressive motility was found, R2 was 0.9623 and 0.9993 for pre-freezing and post-thaw progressive motility, respectively. High amounts of cryoprotective agent (CPA) were observed to impair both sperm capacitation and fertilization. Moreover, the presence of a large number of immotile sperm in the sperm-oocyte co-incubation drop was found to reduce IVF success which could be partially reversed by supplementation using monothioglycerol (MTG) during centrifugation. It was concluded that the efficiency of IVF using cryorecovered mouse sperm in media containing MBCD and GSH can be predicted from sperm progressive motility. High concentrations of CPA and immotile sperm should be mitigated prior to IVF. The optimum IVF method should be selected based on sperm sample volume and sperm parameters.

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