alexa Cytomorphometric Analysis of Non-keratinized Malignant Squamous Cells in Exfoliated Cytosmears of Human Oral Neoplasm
ISSN: 2157-2518

Journal of Carcinogenesis & Mutagenesis
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Research Article

Cytomorphometric Analysis of Non-keratinized Malignant Squamous Cells in Exfoliated Cytosmears of Human Oral Neoplasm

Mohanta A* and Prafulla KM

Department of Zoology, Utkal University, Vani Vihar, Bhubaneshwar, Odisha, India

*Corresponding Author:
Mohanta A
Department of Zoology, Utkal University
Vani Vihar, Bhubaneshwar, Odisha, India
Tel: +919937575859
E- mail: [email protected]

Received date: December 17, 2015; Accepted date: January 08, 2016; Published date: January 12, 2016

Citation: Mohanta A, Prafulla KM (2016) Cytomorphometric Analysis of Non-keratinized Malignant Squamous Cells in Exfoliated Cytosmears of Human Oral Neoplasm. J Carcinog Mutagene 7:247. doi: 10.4172/2157-2518.1000247

Copyright: © 2015 Mohanta A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



Objectives: During the present investigation, a unique type of pleomorphic cytological atypia- named as nonkeratinized malignant squamous cell (NMSC) was frequently observed in the exfoliated cytosmears of the oral cancer patients. Details of these peculiar atypical, pleomorphic cells have not been reported earlier. Therefore, an attempt has been undertaken to find out the practical utility of NMSCs during difficult diagnoses and in early detection of oral squamous cell carcinoma (OSCC) cases through cytomorphometric analysis.
Materials and Methods: In a hospital-based case-control study exfoliated scrape smears were collected from the clinically diagnosed 136 patients suffering from precancerous lesions and cancerous cases and a parallel set of 136 samples were also collected from the non-addicted and non-cancerous healthy individuals considered as control group. Wet fixed smears were stained by adopting Papanicolaou’s staining protocol and counter-stained with Giemsa’s solution. Out of one thousand screened cells, frequently observed NMSCs were scored. Cytomorphometry was done by using computer-assisted Cat Cam 1.30 (1.3 Mega Pixel) Microscope Camera® fitted with hund®-H500 research binocular microscope. The findings were statistically analyzed and interpreted by using software package PAleontological Statistics (PAST)® Version 2.17 with respect to age groups, degree of pathogenicity, oral sites and sexes.
Results: Cytomorphometrically, the cellular parameters (mean length, breadth and area) of the NMSC were found to be in decreased state and nuclear parameters are in increased state. The quantitative parameters of the NMSCs were significantly (p ≤ 0.01) decreased with increasing age groups. Cytomorphometrically, the nuclearcytoplasmic (N/C) ratio of the NMSC was found to be 1:1 in both sexes.
Conclusion: Extreme reduction of cellular diameter and increased nuclear diameter in each NMSC, lead to cellular non-keratinization, hyperchromasia and increased N/C ratios in both sexes indicate the state of malignancy. Thus, the present finding has a practical utility in early detection and diagnosis of the OSCC patients.


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