alexa Cytoprotective Properties of Antioxidant Protein from C
ISSN: 2161-0444

Medicinal Chemistry
Open Access

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Research Article

Cytoprotective Properties of Antioxidant Protein from Curry leaves (Murraya koenigii L.) against Oxidative Stress Induced Damage in Human Erythrocytes

Mylarappa B Ningappa1,2*, Dinesha Ramadas1, Dinesha Nanjegowda1, Kiruthika Balasubramanian1, Khusdeep Chahal3, Sachin Patil4 and Leela Srinivas1

1Adichunchanagiri Biotechnology and Cancer Research Institute, Central Research Laboratory, Adichunchanagiri Institute of Medical Sciences, Balagangadharanatha Nagara, Mandya District, Karnataka, India

2Department of Surgery, University of Pittsburgh, Pittsburgh, PA, USA

3UAB School of Medicine, Huntsville Regional Campus, Huntsville, AL 35801, USA

4Presence Medical Center, University of Illinois and Hospitalist (Christie Clinic), Urbana, IL, USA

*Corresponding Author:
Mylarappa B. Ningappa
Research Associate, Department of Surgery
University of Pittsburgh, Rangos Research Center
530 45th street, Pittsburgh, PA, United States
Tel: 4126573269
E-mail: [email protected]

Received date: December 27, 2015; Accepted date: February 05, 2016; Published date: February 08, 2016

Citation: Ningappa MB, Ramadas D, Nanjegowda D, Balasubramanian K, Chahal K, et al. (2016) Cytoprotective Properties of Antioxidant Protein from Curry leaves (Murraya koenigii L.) against Oxidative Stress Induced Damage in Human Erythrocytes. Med chem 6:075-080. doi:10.4172/2161-0444.1000328

Copyright: © 2016 Ningappa MB, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Oxidative stress induced by reactive oxygen species (ROS) cause lipid peroxidation at the human erythrocyte membrane with subsequent alterations in the ATPases function. In this study, potential cytoprotective activities of previously reported ”Antioxidant Protein from Curry leaves (APC)’’ in erythrocytes against reactive oxygen (ROS) species generated by pro-oxidants in vitro. The APC prevented red blood cell lysis induced by pro-oxidants; Fe: As (2:20 μmole), Hydrogen peroxide (0.2 mM), and tertiary butyl hydroperoxide (1 mM) upto 97.5, 82.5 and 63%, respectively. Further, APC prevented Fe:As induced K+ leakage in red blood cells up to 95%. The inhibition offered by APC on K+ leakage was comparable to inhibition offered by quinine sulphate, a known K+ channel blocker. Interestingly, the APC at dose dependently restored Na+K+ ATPase and Ca2+Mg2+ ATPase activities of erythrocyte membrane when altered by ROS. The restoration of ATPase activity by APC was two times more than standard antioxidants BHA and α-tocopherol. In conclusion, Curry leaves protein antioxidant is an effective antioxidant in preventing membrane damage and associated functions mediated by reactive oxygen species. It can be further developed as an effective bioprotective antioxidant agent to cellular components.

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