alexa Cytotoxic Effect of Astringent Agents on Human Gingival Fibroblasts | OMICS International | Abstract
ISSN: 2247-2452

Oral Health and Dental Management
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Cytotoxic Effect of Astringent Agents on Human Gingival Fibroblasts

Abstract

Aims: During fixed prosthesis fabrication, gingival margin retraction is an important step before making an impression. Astringent agents should provide sufficient free gingival margin displacement, and must be free of systemic and local harmful adverse effects. This study aimed to determine the biocompatibility of three different astringent agents on human gingival fibroblast. Materials and Methods: In order to evaluate cytotoxicity of 25% aluminum chloride, 25% aluminum sulphate and 20% ferric sulphate, in 24 well culture plates containing human gingival fibroblasts, RPMI media, antibiotic and 10% fetal bovine serum was added. Cell cultures were incubated in a CO2 incubator. After 1, 5 and 15 minutes, optical absorbance of each plate was determined by MTT assay. Cytotoxicity of each astringent at 1, 5 and 15 minutes were compared by using Student t-test. A p-value<0.05 was considered as significant level. Results: The cytotoxicity of aluminum chloride at all time periods was significantly greater than other two astringents (p<0.05). At 1 minute application, cytotoxicity of ferric sulphate was significantly lower than aluminum sulphate (p=0.01). At 5 minutes, the effect of ferric sulphate and aluminum sulphate was similar and at 15 minutes, aluminum sulphate had significantly lower cytotoxicity compared to ferric sulphate (p=0.043). Conclusions: At all tested time periods, 25% aluminum chloride exhibited greater cytotoxicity than aluminum sulphate and ferric sulphate. Compared to aluminum sulphate, the cytotoxicity of ferric sulphate was lower at 1 minute, similar at 5 minutes, and greater at 15 minutes.

Keywords

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version