alexa Danggui Buxue Tang, a Chinese Herbal Decoction, Induces Nitric Oxide Production in Cultured Endothelial Cells: Probing Calycosin as a Key Player by Chemical Knock-Out Approach

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Research Article

Danggui Buxue Tang, a Chinese Herbal Decoction, Induces Nitric Oxide Production in Cultured Endothelial Cells: Probing Calycosin as a Key Player by Chemical Knock-Out Approach

Gong AGW1, Lam CTW1, Zhang LML1, Deng JY1, Wang HY1,2, Lin HQ1, Dong TTX1and Tsim KWK1*

1Division of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China

2HKUST Shenzhen Research Institute, Hi-Tech Park, Nanshan, Shenzhen 518000, Guangdong Province, China

*Corresponding Author:
Karl WK Tsim
Professor, Division of Life Science
Center for Chinese Medicine
the Hong Kong University of Science and Technology
Clear Water Bay Road, Hong Kong SAR, China
Tel: 85223587332
E-mail: [email protected]

Received Date: March 9, 2016 Accepted Date: March 29, 2016 Published Date: March 31, 2016

Citation:Gong AGW, Lam CTW, Zhang LML, Deng JY, Wang HY, et al. (2016) Danggui Buxue Tang, a Chinese Herbal Decoction, Induces Nitric Oxide Production in Cultured Endothelial Cells: Probing Calycosin as a Key Player by Chemical Knock-Out Approach. J Res Development S1: 001. doi:10.4172/2311-3278.1000S1-001

Copyright: © 2016 Gong AGW, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

 

Abstract

Danggui Buxue Tang (DBT) is a Chinese herbal decoction prescribed for woman menopausal symptoms. The make-up of DBT is Astragali Radix (AR) and Angelicae Sinensis Radx (ASR) at a weight ratio of 5:1. In cultured endothelial cells, application of DBT induced the production of nitric oxide (NO), the phosphorylations of eNOS and Akt, and the mobilization of intracellular calcium. The chemicals within DBT responsible for the effects in endothelial cells however have never been addressed. Here, we developed a chemical knock-out method in a herbal mixture as to determine the role of individual ingredient(s). Calycosin is the most abundant flavonoid in DBT herbal decoction. By HPLC separation, calycosin was removed at over 97% from DBT decoction, which was named as DBTΔcal. Comparing to authentic DBT, the induction of NO production as well as its signaling responses, triggered by DBTΔcal, in cultured endothelial cells were markedly reduced. In contrast, calycosin alone showed no response in the cultures. Our finding showed a synergistic role of calycosin in the herbal mixture.

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