alexa De Novo Assembly and Analysis of the Testes Transcriptome from the Menhaden, Bervoortia tyrannus | OMICS International | Abstract
ISSN: 2150-3508

Fisheries and Aquaculture Journal
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

De Novo Assembly and Analysis of the Testes Transcriptome from the Menhaden, Bervoortia tyrannus

Frank J Zadlock IV1*, Satshil B Rana1, Zain A Alvi1, Ziping Zhang2, Wyatt Murphy1 and Carolyn S Bentivegna3

1Department of Biological Science, Seton Hall University, South Orange, New Jersey, USA

2College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, China

3Department of Chemistry and Biochemistry, Seton Hall University, South Orange, New Jersey, USA

*Corresponding Author:
Frank J Zadlock IV
Department of Biological Science, Seton Hall University
South Orange, New Jersey, USA
Tel: (973) 313-6146
E-mail: [email protected]

Received date: November 13, 2016; Accepted date: December 05, 2016; Published date: December 08, 2016

Citation: Zadlock FJ, Rana SB, Alvi ZA, Zhang Z, Murphy W, et al. (2016) De Novo Assembly and Analysis of the Testes Transcriptome from the Menhaden, Bervoortia tyrannus. Fish Aqua J 8:186. doi:10.4172/2150-3508.1000186

Copyright: © 2016 Zadlock FJ, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Background: The menhaden, Bervoortia tyrannus, is one of the most important fish within the oceanic ecosystem and a crucial species supporting major fisheries along the Atlantic and Gulf coasts. However, little is known about menhaden from a genetic aspect. The objective of this project is to apply high throughput sequencing to the testes of menhaden to provide the genetic tools required to further study their population dynamics

Result: We applied Illumina Next Seq 500 technology to two different testes and used Velvet/Oases to perform the de novo assembly that resulted in the construction of 254,462 contigs. Applying BLASTX to annotate the contigs against the non-redundant protein database resulted in 46.89% matches. To validate the accuracy of the assembly, the reads were mapped back to transcripts (RMBT) with a percentage of 87.83%. To experimentally verify the assembly results, primers were designed based on the assembled transcriptome, and PCR products were verified by Sanger sequencing. To enhance the functional categorization of the annotated contigs, they were further classified using Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Clusters of Orthologous Groups (COG) databases.

Conclusion: This research is the first report of an annotated overview of the testes transcriptomes in B. tyrannus, resulting in the most comprehensive genetic resource available for menhaden to date. This work can provide a repository for future gene expression analysis, functional studies, and reproductive investigations in B. tyrannus. This will enhance the capabilities of population monitoring and can be used as a benchmark in comparative studies in other fish models. Overall, this research will open new opportunities and bring new insights for researchers studying B. tyrannus.

Keywords

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7