alexa Degradation of Diesel, a Component of the Explosive ANFO, By Bacteria Selected From an Open Cast Coal Mine in La Guajira, Colombia | OMICS International | Abstract
ISSN: 2155-9821

Journal of Bioprocessing & Biotechniques
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Research Article

Degradation of Diesel, a Component of the Explosive ANFO, By Bacteria Selected From an Open Cast Coal Mine in La Guajira, Colombia

Jenny Dussán* and Mónica Numpaque
Centro de Investigaciones Microbiológicas-CIMIC, Departamento de Ciencias Biológicas, Universidad de los Andes, Bogotá, Colombia
Corresponding Author : Jenny Dussán
Centro de Investigaciones Microbiológicas- CIMIC
Departamento de Ciencias Biológicas
Universidad de los Andes, Bogotá,Colombia
Tel: (571)3394949
E-mail: [email protected]
Received May 17, 2012; Accepted September 24, 2012; Published October 04, 2012
Citation: Dussán J, Numpaque M (2012) Degradation of Diesel, a Component of the Explosive ANFO, By Bacteria Selected From an Open Cast Coal Mine in La Guajira, Colombia. J Bioprocess Biotech 2:126. doi: 10.4172/2155-9821.1000126
Copyright: © 2012 Dussán J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Open cast coal mining operations involve the use of the explosive Ammonium Nitrate Fuel Oil (ANFO) for detonation processes. Five bacterial strains belonged to Pseudomonas sp and Pseudomonas stutzeri were isolated from an open cast coal mine located in La Guajira, Colombia. Degradation of the second component of ANFO, which is diesel, by the five isolates and by a consortium was evaluated. The biodegradation of diesel was determined by gas chromatography with a flame ionization detector. Biodegradation Efficiency (BE) was 96% for the consortium; individual strains had levels between 10-95% of BE. Analysis for the presence and expression of the alkane monooxygenase gene involved in the degradation of diesel was evidenced in two strains showing band size products between 500-600 bp. Results suggest that these bacteria are candidates for diesel bioremediation. 

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