Dehydrogenase Activity in the Brain of Fluoride and Aluminium Induced Wistar Rats
|Akinrinade ID1*, Ogundele OM2, Memudu AE1 and Dare BJ1|
|1Bingham University, Department of Anatomy, Karu, Nigeria|
|2Afe Babalola University, Biology Department, Ado- Ekiti, Nigeria|
|*Corresponding Author :||Akinrinade I.D
Department of Anatomy, Karu, Nigeria
E-mail: [email protected]
|Received November 08, 2012; Accepted December 17, 2012; Published December 19, 2012|
|Citation: Akinrinade ID, Ogundele OM, Memudu AE, Dare BJ (2013) Dehydrogenase Activity in the Brain of Fluoride and Aluminium Induced Wistar Rats. Biol Syst Open Access 2:110. doi:10.4172/BSO.1000110|
|Copyright: ©2013 Akinrinade ID, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
Dehydrogenases are cellular enzymes usually used as indicators of changes in cell activity and morphology; this includes metabolic processes such as structural differentiation, cell migration, cellular damage and even cell death; hence, assay of enzymes as Lactate dehydrogenase and Glucose 6 phosphate dehydrogenase could provide evidence for the role of some compounds in the stimulation of oxidative stress, cellular damage and eventually cell death.
In this study, examination of Lactate dehydrogenase and Glucose 6 phosphate dehydrogenase in the Prefrontal cortex of Aluminium and Fluoride induced wistar rats was done by administering sodium fluoride (10 mg/kg) and Aluminium Chloride (200 mg/kg). The study employed assay of glucose-6-phospahte dehydrogenase (G6PDH) and lactate dehydrogenase (LDH) levels to determine possible oxidative stress and cellular damage. Results showed alterations in the enzyme levels of G6PDH and LDH indicating a high level of dehydrogenase activity in the treatment groups. This shows that Fluoride and Aluminium have a high potential of causing excitotoxicity, oxidative stress and eventually cellular damage.