Delayed Post Hatch Feeding Affects Performance, Intestinal Morphology and Expression Pattern of Nutrient Transporter Genes in Egg Type ChickensAnil S Shinde, Akshat Goel, Manish Mehra, Jaydeep Rokade, Pragya Bhadauria, Asit B. Mandal and Subrat K. Bhanja*
Central Avian Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India
- *Corresponding Author:
- Subrat K. Bhanja
Central Avian Research Institute
Izatnagar, Bareilly, Uttar Pradesh, India
E-mail: [email protected]
Received date: March 25, 2015; Accepted date: April 17, 2015; Published date: April 27, 2015
Citation: Shinde AS, Goel A, Mehra M, Rokade J, Bhadauria P, et al. (2015) Delayed Post Hatch Feeding Affects Performance, Intestinal Morphology and Expression Pattern of Nutrient Transporter Genes in Egg Type Chickens. J Nutr Food Sci 5:372. doi: 10.4172/2155-9600.1000372
Copyright: © 2015 Shinde AS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Effect of post-hatch (PH) feed deprivation (FD) for 6, 12, 24 and 36 hrs., on the performance, gut development and differential expression of nutrient transporter genes in egg type chickens (crosses of White Leghorn) was studied. Significant decrease (P=0.001) in yolk utilization (in all the FD chicks) and relative weight of gizzard, intestine, liver and pancreas was observed in 24 and 36 hrs FD chicks. The 36 hrs FD chicks recorded lower (P=0.001) body weight, lower feed intake and inferior FCR, decreased serum glucose, but higher cholesterol and uric acid level than the control (immediate fed group) or 6 hrs FD chicks. Villi height in duodenum, jejunum and ileum decreased (P=0.001) but villi width increased (P=0.001) with increase in FD period and significant changes were observed particularly in 24 or 36 hrs FD chicks. Relative expression of Cdx gene decreased with age of the bird and the feed restriction period. Expression of SGLT, FABP gene was not associated with the feed deprivation period, while that of EAAT3 gene increased in 24 or 36 hrs FD chicks. No difference was observed in bursa, spleen and thymus weight. In vivo humoral and cellular immune response was significantly better in chicks FD for 6, 12 and 24 hrs than control and 36 hrs FD chicks. Expression of immunity related genes IL-6 and TLR-2 increased as the FD period increased. It may concluded that PH feed deprival for first 12 hrs did not affect growth performance, intestinal morphology and immune response but feed withdrawal for 24 or 36 hrs adversely affect the intestinal morphology and few nutrient transporter genes expression in egg type chickens.