alexa Demonstration of Muscle Fibre Types Using Masson Trichr

Journal of Molecular Histology & Medical Physiology
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Research Article

Demonstration of Muscle Fibre Types Using Masson Trichrome Stain from Deltoideus Muscles of One-humped Camel (Camelus Dromedarius)

Hena SA1*, Sonfada ML1, Shehu SA1, Jibir M2, Bello A1 and Wiam IM3

1Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria

2Department of Animal Science, Faculty of Agriculture, Usmanu Danfodiyo University, Sokoto, Nigeria

3Department of Veterinary Anatomy, Faculty of Veterinary Medicine, University of Maiduguri, Nigeria

*Corresponding Author:
Hena SA
Department of Veterinary Anatomy
Faculty of Veterinary Medicine
Usmanu Danfodiyo University
Sokoto, Nigeria
Tel: 2348060524623
E-mail: [email protected]

Received Date: November 30, 2016; Accepted Date: January 11, 2017; Published Date: January 20, 2017

Citation: Hena SA, Sonfada ML, Shehu SA, Jibir M, Bello A, et al. (2017) Demonstration of Muscle Fibre Types Using Masson Trichrome Stain from Deltoideus Muscles of One-humped Camel (Camelus Dromedarius). J Mol Histol Med Physiol 2: 111.

Copyright: © 2017 Hena SA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

This study involved the use of 25 male camels (Camelus dromedarius) each within the ages of 6 months, 1 year, 3 years, 5 years and 7 years, which were purchased from Sokoto Municipal Modern abattoir. 1 cm2 of each deltoideus muscle sample was taken from the middle part of the muscle belly and fixed in Bouin’s solution for histological preparation using Masson trichrome according to standard procedure; the obtained slides were viewed using a miscroscope using different magnifications. The Masson trichrome stain revealed some collagen connective tissue fibres, and varying proportions and distribution patterns of muscle fibre types I, IIA and IIB. This study could help in advancing the course of knowledge in this study field and as well guide in clinical practice more especially during evaluation of muscular procedures and wound healing.

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